2vem: Difference between revisions

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==Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties==
==Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties==
<StructureSection load='2vem' size='340' side='right' caption='[[2vem]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='2vem' size='340' side='right'caption='[[2vem]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2vem]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trybb Trybb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VEM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VEM FirstGlance]. <br>
<table><tr><td colspan='2'>[[2vem]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VEM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VEM FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BBR:(3-BROMO-2-OXO-PROPOXY)PHOSPHONIC+ACID'>BBR</scene>, <scene name='pdbligand=TBU:TERTIARY-BUTYL+ALCOHOL'>TBU</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1iih|1iih]], [[1kv5|1kv5]], [[1tpf|1tpf]], [[1trd|1trd]], [[1tsi|1tsi]], [[1tti|1tti]], [[1ttj|1ttj]], [[2j24|2j24]], [[2j27|2j27]], [[2v2c|2v2c]], [[2v2d|2v2d]], [[2v2h|2v2h]], [[3tim|3tim]], [[1ag1|1ag1]], [[1dkw|1dkw]], [[1iig|1iig]], [[1ml1|1ml1]], [[1mss|1mss]], [[1mtm|1mtm]], [[1tpd|1tpd]], [[1tpe|1tpe]], [[1tri|1tri]], [[2v0t|2v0t]], [[2v5l|2v5l]], [[4tim|4tim]], [[5tim|5tim]], [[6tim|6tim]], [[2vei|2vei]], [[2vek|2vek]], [[2vel|2vel]], [[2ven|2ven]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BBR:(3-BROMO-2-OXO-PROPOXY)PHOSPHONIC+ACID'>BBR</scene>, <scene name='pdbligand=TBU:TERTIARY-BUTYL+ALCOHOL'>TBU</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vem OCA], [https://pdbe.org/2vem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vem RCSB], [https://www.ebi.ac.uk/pdbsum/2vem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vem ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vem OCA], [http://pdbe.org/2vem PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vem RCSB], [http://www.ebi.ac.uk/pdbsum/2vem PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vem ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TPIS_TRYBB TPIS_TRYBB]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ve/2vem_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ve/2vem_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
   </jmolCheckbox>
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==See Also==
==See Also==
*[[Triose Phosphate Isomerase|Triose Phosphate Isomerase]]
*[[Triose phosphate isomerase 3D structures|Triose phosphate isomerase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Triose-phosphate isomerase]]
[[Category: Large Structures]]
[[Category: Trybb]]
[[Category: Trypanosoma brucei brucei]]
[[Category: Alahuhta, M]]
[[Category: Alahuhta M]]
[[Category: Augustyns, K]]
[[Category: Augustyns K]]
[[Category: Casteleijn, M G]]
[[Category: Casteleijn MG]]
[[Category: El-Sayed, I]]
[[Category: El-Sayed I]]
[[Category: Kemmer, C]]
[[Category: Kemmer C]]
[[Category: Neubauer, P]]
[[Category: Neubauer P]]
[[Category: Salin, M]]
[[Category: Salin M]]
[[Category: Wierenga, R K]]
[[Category: Wierenga RK]]
[[Category: Binding pocket]]
[[Category: Engineering]]
[[Category: Enzyme]]
[[Category: Fatty acid biosynthesis]]
[[Category: Gluconeogenesis]]
[[Category: Glycolysis]]
[[Category: Glycosome]]
[[Category: Isomerase]]
[[Category: Lipid synthesis]]
[[Category: Monomeric]]
[[Category: Pentose shunt]]
[[Category: Substrate specificity]]
[[Category: Tim]]
[[Category: Tim barrel]]
[[Category: Triosephosphate isomerase]]
Retrieved from "https://proteopedia.org/2vem"