6mis: Difference between revisions
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New page: '''Unreleased structure''' The entry 6mis is ON HOLD Authors: Yongqing, T., Wilmann, P.G., Pike, R.N., Wijeyewickrema, L.C. Description: Native ananain in complex with E-64 [[Category:... |
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==Native ananain in complex with E-64== | |||
<StructureSection load='6mis' size='340' side='right'caption='[[6mis]], [[Resolution|resolution]] 1.98Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6mis]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ananas_comosus Ananas comosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MIS FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mis OCA], [https://pdbe.org/6mis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mis RCSB], [https://www.ebi.ac.uk/pdbsum/6mis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mis ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ANAN_ANACO ANAN_ANACO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ananain (EC 3.4.22.31) accounts for less than 10% of the total enzyme in the crude pineapple stem extract known as bromelain, yet yields the majority of the proteolytic activity of bromelain. Despite a high degree of sequence identity between ananain and stem bromelain, the most abundant bromelain cysteine protease, ananain displays distinct chemical properties, substrate preference and inhibitory profile compared to stem bromelain. A tripeptidyl substrate library (REPLi) was used to further characterize the substrate specificity of ananain and identified an optimal substrate for cleavage by ananain. The optimal tripeptide, PLQ, yielded a high kcat/Km value of 1.7 x 106 M(-1)s(-1), with cleavage confirmed to occur after the Gln residue. Crystal structures of unbound ananain and an inhibitory complex of ananain and E-64, solved at 1.73 and 1.98A, respectively, revealed a geometrically flat and open S1 subsite for ananain. This subsite accommodates diverse P1 substrate residues, while a narrow and deep hydrophobic pocket-like S2 subsite would accommodate a non-polar P2 residue, such as the preferred Leu residue observed in the specificity studies. A further illustration of the atomic interactions between E-64 and ananain explains the high inhibitory efficiency of E-64 toward ananain. These data reveal the first in depth structural and functional data for ananain and provide a basis for further study of the natural properties of the enzyme. | |||
Determination of the crystal structure and substrate specificity of ananain.,Yongqing T, Wilmann PG, Pan J, West ML, Brown TJ, Mynott T, Pike RN, Wijeyewickrema LC Biochimie. 2019 Jul 12. pii: S0300-9084(19)30202-0. doi:, 10.1016/j.biochi.2019.07.011. PMID:31306685<ref>PMID:31306685</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6mis" style="background-color:#fffaf0;"></div> | ||
[[Category: Wijeyewickrema | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Ananas comosus]] | |||
[[Category: Large Structures]] | |||
[[Category: Pike RN]] | |||
[[Category: Wijeyewickrema LC]] | |||
[[Category: Wilmann PG]] | |||
[[Category: Yongqing T]] | |||
Latest revision as of 06:32, 11 October 2023
Native ananain in complex with E-64
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