Transketolase: Difference between revisions

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== Function ==

'''Transketolase''' (TKT) catalyzes two opposite reactions. The synthesis of sedoheptulose-7-P in the pentose phosphate pathway using thiamine diphosphate (TPP) as co-factor; and the conversion of sedoheptulose-7-P and glyceraldehyde-3-P to aldose and ketose in the Calvin cycle<ref>PMID:8369276</ref>.

'''Transketolase''' (TKT) catalyzes two opposite reactions. The synthesis of sedoheptulose-7-P in the pentose phosphate pathway using thiamine diphosphate (TPP) as co-factor; and the conversion of sedoheptulose-7-P and glyceraldehyde-3-P to aldose and ketose in the [[Calvin cycle]]<ref>PMID:8369276</ref>. '''Split-gene TKT''' is found in the hyperthermophilic bacterium ''Carboxydothermus hydrogenoformans'' and is reconstituted from 2 separate polypeptide chains<ref>PMID:33193259</ref>.

== Structural highlights ==

The <scene name='46/466534/Cv/5'>active site of TKT contains a ligand-cofactor adduct</scene>.<ref>PMID:17914867</ref> <scene name='46/466534/Cv/6'>Ca coordination site</scene>.

The <scene name='46/466534/Cv/7'>active site of TKT contains a ligand-cofactor adduct</scene>.<ref>PMID:17914867</ref> Water molecules are shown as red spheres. <scene name='46/466534/Cv/8'>Ca coordination site</scene>.

~~</StructureSection>~~

== 3D Structures of transketolase ==

~~Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}~~

[[Transketolase 3D structures]]

~~{{#tree:id=OrganizedByTopic|openlevels=0|~~

</StructureSection>

* Transketolase

**[[1trk]] – yTKT – yeast

**[[1ay0]] – yTKT (mutant)

**[[2e6k]] – TKT – ''Thermus thermophilus''

**[[3hyl]] – BaTKT – ''Bacillus anthracis''

**[[3kom]] – TKT (mutant) – ''Francisella tularensis''

**[[3l84]] – CjTKT – ''Campylobacter jejuni''

**[[3rim]] – TKT – ''Mycobacterium tuberculosis''

**[[3uk1]] – BtTKT – ''Burkholderia thailandensis''

**[[4c7v]] - LsTKT – ''Lactobacillus salivarius''

**[[4xeu]] - TKT – ''Pseudomonas aeruginosa''

**[[5i4i]], [[5xry]] - PsTKT – ''Pichia stipitis''

**[[5hgx]], [[5i5g]] - PsTKT (mutant)

**[[5nd6]] - CrTKT – ''Chlamydomonas reinhardtii''

**[[~~5vrb]] - TKT – ''Neisseria gonorrhoeae'' ~~

*Transketolase ~~binary complexes~~

**[[3m49]] – BaTKT + TPP

**[[3mos]]~~, [[3ooy~~]~~] – hTKT + TPP - human ~~

**[[4kxy]] - hTKT + TPP analog

**[[1tka]], [[1tkb]], [[1tkc]] – yTKT + TPP analog

**[[1gpu]] - yTKT 1 + TPP derivative

**[[1itz]] - TKT + TPP – maize

**[[1qgd]] - EcTKT + TPP – ''Escherichia coli''

**[[5hht]] - EcTKT (mutant) + TDP

**[[1r9j]] - TKT + TPP – ''Leishmania mexicana''

**[[4c7x]] - LsTKT + TPP

**[[5hyv]], [[5x56]], [[5xuf]], [[5xvt]] - PsTKT + TPP

**[[5xsa]], [[5xsb]], [[5xsm]], [[5xt0]], [[5xt4]], [[5xtv]], [[5xtx]] - PsTKT + TPP intermediate

**[[5xtl]] - PsTKT + pyrimidine derivative

**[[5nd5]] - CrTKT + TPP

*Transketolase ternary complexes

**[[3m7i]], [[3m6l]] – CjTKT + TPP + ribose 5-P

**[[3m34]] - CjTKT + TPP + Ca

**[[1ngs]] - yTKT + TPP + Ca + erythrose-4-P

**[[2r5n]] – EcTKT 1 + TPP + ribose 5-P

**[[3upt]],[[5xqa]] - BtTKT + TPP + ribose 5-P

**[[2r8o]], [[5xqk]] - EcTKT 1 + TPP + xylulose 5-P

**[[2r8p]] - EcTKT 1 + TPP + fructose 6-P

**[[4kxu]] - hTKT + TPP + sorbitol 6-P

**[[4kxv]], [[4kxw]] - hTKT + TPP + xylitol 5-P

**[[4kxx]] - hTKT + TPP + seduheptulose 7-P

**[[5hje]] - PsTKT + TPP + seduheptulose 7-P

**[[5i51]], [[5xrv]], [[5xu2]] - PsTKT + TPP + fructose 6-P

**[[5xps]], [[5xu9]] - PsTKT + TPP + erythrose 4-P

**[[5i5e]] - PsTKT (mutant) + TPP + xylulose 5-P

}}

== References ==

[[Category:Topic Page]]

Transketolase: Difference between revisions

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@@ Line 1: / Line 1: @@
 <StructureSection load='' size='350' side='right' caption='E. coli transketolase 1 dimer complex with xylulose-5-phosphate-thiamine diphosphate adduct, ethylene glycol and Ca+2 ion (green)  (PDB code [[2r8o]]).' scene='46/466534/Cv/1'>
 == Function ==
-'''Transketolase''' (TKT) catalyzes two opposite reactions.  The synthesis of sedoheptulose-7-P in the pentose phosphate pathway using thiamine diphosphate (TPP) as co-factor; and the conversion of sedoheptulose-7-P and glyceraldehyde-3-P to aldose and ketose in the Calvin cycle<ref>PMID:8369276</ref>.
+'''Transketolase''' (TKT) catalyzes two opposite reactions. The synthesis of sedoheptulose-7-P in the pentose phosphate pathway using thiamine diphosphate (TPP) as co-factor; and the conversion of sedoheptulose-7-P and glyceraldehyde-3-P to aldose and ketose in the [[Calvin cycle]]<ref>PMID:8369276</ref>. '''Split-gene TKT''' is found in the hyperthermophilic bacterium ''Carboxydothermus hydrogenoformans'' and is reconstituted from 2 separate polypeptide chains<ref>PMID:33193259</ref>.
 == Structural highlights ==
-The <scene name='46/466534/Cv/5'>active site of TKT contains a ligand-cofactor adduct</scene>.<ref>PMID:17914867</ref> <scene name='46/466534/Cv/6'>Ca coordination site</scene>.
+The <scene name='46/466534/Cv/7'>active site of TKT contains a ligand-cofactor adduct</scene>.<ref>PMID:17914867</ref> Water molecules are shown as red spheres. <scene name='46/466534/Cv/8'>Ca coordination site</scene>.
-</StructureSection>
 == 3D Structures of transketolase ==
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+[[Transketolase 3D structures]]
-{{#tree:id=OrganizedByTopic|openlevels=0|
+</StructureSection>
-* Transketolase
-**[[1trk]] – yTKT – yeast
-**[[1ay0]] – yTKT (mutant)
-**[[2e6k]] – TKT – ''Thermus thermophilus''
-**[[3hyl]] – BaTKT – ''Bacillus anthracis''
-**[[3kom]] – TKT (mutant) – ''Francisella tularensis''
-**[[3l84]] – CjTKT – ''Campylobacter jejuni''<br />
-**[[3rim]] – TKT – ''Mycobacterium tuberculosis''<br />
-**[[3uk1]] – BtTKT – ''Burkholderia thailandensis''<br />
-**[[4c7v]] - LsTKT – ''Lactobacillus salivarius''<br />
-**[[4xeu]] - TKT – ''Pseudomonas aeruginosa''<br />
-**[[5i4i]], [[5xry]] - PsTKT – ''Pichia stipitis''<br />
-**[[5hgx]], [[5i5g]] - PsTKT (mutant)<br />
-**[[5nd6]] - CrTKT – ''Chlamydomonas reinhardtii''<br />
-**[[5vrb]] - TKT – ''Neisseria gonorrhoeae''<br />
-*Transketolase binary complexes
-**[[3m49]] – BaTKT + TPP
-**[[3mos]], [[3ooy]] – hTKT + TPP - human<br />
-**[[4kxy]] - hTKT + TPP analog<br />
-**[[1tka]], [[1tkb]], [[1tkc]] – yTKT + TPP analog
-**[[1gpu]] - yTKT 1 + TPP derivative
-**[[1itz]] - TKT + TPP – maize
-**[[1qgd]] - EcTKT + TPP – ''Escherichia coli''<br />
-**[[5hht]] - EcTKT (mutant) + TDP <br />
-**[[1r9j]] - TKT + TPP – ''Leishmania mexicana''<br />
-**[[4c7x]] - LsTKT + TPP <br />
-**[[5hyv]], [[5x56]], [[5xuf]], [[5xvt]] - PsTKT + TPP <br />
-**[[5xsa]], [[5xsb]], [[5xsm]], [[5xt0]], [[5xt4]], [[5xtv]], [[5xtx]] - PsTKT + TPP intermediate<br />
-**[[5xtl]] - PsTKT + pyrimidine derivative <br />
-**[[5nd5]] - CrTKT + TPP <br />
-*Transketolase ternary complexes
-**[[3m7i]], [[3m6l]] – CjTKT + TPP + ribose 5-P
-**[[3m34]] - CjTKT + TPP + Ca
-**[[1ngs]] - yTKT + TPP + Ca + erythrose-4-P
-**[[2r5n]] – EcTKT 1 + TPP + ribose 5-P<br />
-**[[3upt]],[[5xqa]] - BtTKT + TPP + ribose 5-P<br />
-**[[2r8o]], [[5xqk]] - EcTKT 1 + TPP + xylulose 5-P
-**[[2r8p]] - EcTKT 1 + TPP + fructose 6-P<br />
-**[[4kxu]] - hTKT + TPP + sorbitol 6-P<br />
-**[[4kxv]], [[4kxw]] - hTKT + TPP + xylitol 5-P<br />
-**[[4kxx]] - hTKT + TPP + seduheptulose 7-P<br />
-**[[5hje]] - PsTKT + TPP + seduheptulose 7-P<br />
-**[[5i51]], [[5xrv]], [[5xu2]] - PsTKT + TPP + fructose 6-P<br />
-**[[5xps]], [[5xu9]] - PsTKT + TPP + erythrose 4-P<br />
-**[[5i5e]] - PsTKT (mutant) + TPP + xylulose 5-P<br />
-}}
 == References ==
 <references/>
 [[Category:Topic Page]]