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[[Image:2qkv.jpg|left|200px]]


{{Structure
==Crystal Structure of the C645S Mutant of the 5th PDZ Domain of InaD==
|PDB= 2qkv |SIZE=350|CAPTION= <scene name='initialview01'>2qkv</scene>, resolution 1.550&Aring;
<StructureSection load='2qkv' size='340' side='right'caption='[[2qkv]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[2qkv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QKV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QKV FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
|GENE= inaD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qkv OCA], [https://pdbe.org/2qkv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qkv RCSB], [https://www.ebi.ac.uk/pdbsum/2qkv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qkv ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=[[2qkt|2QKT]], [[2qku|2QKU]]
== Function ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qkv OCA], [http://www.ebi.ac.uk/pdbsum/2qkv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qkv RCSB]</span>
[https://www.uniprot.org/uniprot/INAD_DROME INAD_DROME] Involved in the negative feedback regulation of the light-activated signaling cascade in photoreceptors through a calcium-mediated process. Interacts with tetrapeptide ligand located in C-terminal sequence of 3 key components of the visual cascade, tethering them and forming a macromolecular signaling phototransduction complex.<ref>PMID:7826638</ref> <ref>PMID:11342563</ref>
}}
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
'''Crystal Structure of the C645S Mutant of the 5th PDZ Domain of InaD'''
Check<jmol>
 
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qk/2qkv_consurf.spt"</scriptWhenChecked>
==Overview==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
The INAD scaffold organizes a multiprotein complex that is essential for proper visual signaling in Drosophila photoreceptor cells. Here we show that one of the INAD PDZ domains (PDZ5) exists in a redox-dependent equilibrium between two conformations--a reduced form that is similar to the structure of other PDZ domains, and an oxidized form in which the ligand-binding site is distorted through formation of a strong intramolecular disulfide bond. We demonstrate transient light-dependent formation of this disulfide bond in vivo and find that transgenic flies expressing a mutant INAD in which PDZ5 is locked in the reduced state display severe defects in termination of visual responses and visually mediated reflex behavior. These studies demonstrate a conformational switch mechanism for PDZ domain function and suggest that INAD behaves more like a dynamic machine rather than a passive scaffold, regulating signal transduction at the millisecond timescale through cycles of conformational change.
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==About this Structure==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qkv ConSurf].
2QKV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QKV OCA].  
<div style="clear:both"></div>
 
== References ==
==Reference==
<references/>
Dynamic scaffolding in a g protein-coupled signaling system., Mishra P, Socolich M, Wall MA, Graves J, Wang Z, Ranganathan R, Cell. 2007 Oct 5;131(1):80-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17923089 17923089]
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Ranganathan, R.]]
[[Category: Ranganathan R]]
[[Category: Socolich, M.]]
[[Category: Socolich M]]
[[Category: membrane]]
[[Category: pdz domain]]
[[Category: peptide binding protein]]
[[Category: scaffolding protein]]
[[Category: sensory transduction]]
[[Category: vision]]
 
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Retrieved from "https://proteopedia.org/2qkv"