5oxx: Difference between revisions
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== | ==Crystal structure of NeqN/NeqC complex from Nanoarcheaum equitans at 1.7A== | ||
<StructureSection load='5oxx' size='340' side='right' caption='[[5oxx]], [[Resolution|resolution]] 1.74Å' scene=''> | <StructureSection load='5oxx' size='340' side='right'caption='[[5oxx]], [[Resolution|resolution]] 1.74Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5oxx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OXX OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[5oxx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nanoarchaeum_equitans_Kin4-M Nanoarchaeum equitans Kin4-M]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OXX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OXX FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.74Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oxx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oxx OCA], [https://pdbe.org/5oxx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oxx RCSB], [https://www.ebi.ac.uk/pdbsum/5oxx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oxx ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q74N74_NANEQ Q74N74_NANEQ] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Split inteins are expressed as two separated subunits (N-intein and C-intein) fused to the corresponding exteins. The specific association of both intein subunits precedes protein splicing, which results in excision of the intein subunits and in ligation, by a peptide bond, of the concomitant exteins. Catalytically active intein precursors are typically too reactive for crystallization or even isolation. Neq pol is the trans-intein of the B-type DNA polymerase I split gene from hyperthermophile Nanoarchaeum equitans. We have determined the crystal structures of both the isolated NeqN and the complex of NeqN and NeqC subunits carrying the wild-type sequences, including the essential catalytic residues Ser1 and Thr+1, in addition to seven and three residues of the N- and C-exteins, respectively. These structures provide detailed information on the unique oxyester chemistry of the splicing mechanism of Neq pol and of the extensive rearrangements that occur in NeqN during the association step. | |||
Structural Insights into Subunits Assembly and the Oxyester Splicing Mechanism of Neq pol Split Intein.,Gordo V, Aparicio D, Perez-Luque R, Benito A, Vilanova M, Uson I, Fita I, Ribo M Cell Chem Biol. 2018 Jul 19;25(7):871-879.e2. doi:, 10.1016/j.chembiol.2018.04.008. Epub 2018 May 10. PMID:29754955<ref>PMID:29754955</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5oxx" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Nanoarchaeum equitans Kin4-M]] | ||
[[Category: | [[Category: Aparicio D]] | ||
[[Category: | [[Category: Fita I]] | ||
[[Category: | [[Category: Perez-Luque R]] | ||
[[Category: | [[Category: Ribo M]] | ||
Latest revision as of 01:28, 28 December 2023
Crystal structure of NeqN/NeqC complex from Nanoarcheaum equitans at 1.7A
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