2qzt: Difference between revisions

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[[Image:2qzt.jpg|left|200px]]


{{Structure
==Crystal Structure of Sterol Carrier Protein 2 Like 2 (SCP2-L2) from Aedes Aegypti==
|PDB= 2qzt |SIZE=350|CAPTION= <scene name='initialview01'>2qzt</scene>, resolution 1.700&Aring;
<StructureSection load='2qzt' size='340' side='right'caption='[[2qzt]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
|SITE= <scene name='pdbsite=AC1:Plm+Binding+Site+For+Residue+A+300'>AC1</scene>, <scene name='pdbsite=AC2:Plm+Binding+Site+For+Residue+B+301'>AC2</scene> and <scene name='pdbsite=AC3:Plm+Binding+Site+For+Residue+A+302'>AC3</scene>
== Structural highlights ==
|LIGAND= <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>
<table><tr><td colspan='2'>[[2qzt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aedes_aegypti Aedes aegypti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QZT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QZT FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
|GENE= Sterol Carrier Protein 2 Like 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7159 Aedes aegypti])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qzt OCA], [https://pdbe.org/2qzt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qzt RCSB], [https://www.ebi.ac.uk/pdbsum/2qzt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qzt ProSAT]</span></td></tr>
|RELATEDENTRY=[[1pz4|1PZ4]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qzt OCA], [http://www.ebi.ac.uk/pdbsum/2qzt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qzt RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/Q0GY13_AEDAE Q0GY13_AEDAE]  
 
== Evolutionary Conservation ==
'''Crystal Structure of Sterol Carrier Protein 2 Like 2 (SCP2-L2) from Aedes Aegypti'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qz/2qzt_consurf.spt"</scriptWhenChecked>
Mosquito sterol carrier protein-2 (AeSCP-2) and sterol carrier protein-2-like2 (AeSCP-2L2) are members of the SCP-2 protein family with similar expression profiles in the mosquito life cycle. In an effort to understand how lipids can be transported by different SCP-2 proteins, the three-dimensional crystal structure of AeSCP-2L2 was solved at 1.7 A resolution. AeSCP-2L2 forms a dimer and binds three fatty acids, one of which resides in a position within the internal cavity at a right angle to the others. This first report of ligand-bound dimerized protein in the SCP-2 protein family indicates that the family has a much more divergent mode of interaction with ligands than previously reported. The potential function of AeSCP-2L2 was investigated via in vivo incorporation of [(3)H]cholesterol and [(3)H]palmitic acid. Overexpression of AeSCP-2L2 in mosquito cells leads to an increased uptake of free fatty acid, whereas knockdown of AeSCP-2L2 in adult females decreases the accumulation of free fatty acid in the fat body from a blood meal. In contrast, overexpression or knockdown of AeSCP-2L2 has no effect on cholesterol uptake. Our results suggest that the main function of AeSCP-2L2 is as a general intracellular fatty acid carrier, as opposed to having a dedicated role in cholesterol transport.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
2QZT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aedes_aegypti Aedes aegypti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QZT OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qzt ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
Three-dimensional structure/function analysis of SCP-2-like2 reveals differences among SCP-2 family members., Dyer DH, Wessely V, Forest KT, Lan Q, J Lipid Res. 2008 Mar;49(3):644-53. Epub 2007 Dec 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18084051 18084051]
</StructureSection>
[[Category: Aedes aegypti]]
[[Category: Aedes aegypti]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Dyer, D H.]]
[[Category: Dyer DH]]
[[Category: Forest, K T.]]
[[Category: Forest KT]]
[[Category: Lan, Q.]]
[[Category: Lan Q]]
[[Category: cholesterol]]
[[Category: fatty acid]]
[[Category: lipid transport]]
[[Category: mosquito]]
[[Category: palmitic acid]]
[[Category: sterol carrier]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:55:01 2008''
Retrieved from "https://proteopedia.org/2qzt"