6i2n: Difference between revisions
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==Helical RNA-bound Hantaan virus nucleocapsid== | |||
<SX load='6i2n' size='340' side='right' viewer='molstar' caption='[[6i2n]], [[Resolution|resolution]] 3.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6i2n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hantaan_hantavirus Hantaan hantavirus] and [http://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6I2N FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6i2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i2n OCA], [http://pdbe.org/6i2n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i2n RCSB], [http://www.ebi.ac.uk/pdbsum/6i2n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i2n ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Negative-strand RNA viruses condense their genome into helical nucleocapsids that constitute essential templates for viral replication and transcription. The intrinsic flexibility of nucleocapsids usually prevents their full-length structural characterisation at high resolution. Here, we describe purification of full-length recombinant metastable helical nucleocapsid of Hantaan virus (Hantaviridae family, Bunyavirales order) and determine its structure at 3.3 A resolution by cryo-electron microscopy. The structure reveals the mechanisms of helical multimerisation via sub-domain exchanges between protomers and highlights nucleotide positions in a continuous positively charged groove compatible with viral genome binding. It uncovers key sites for future structure-based design of antivirals that are currently lacking to counteract life-threatening hantavirus infections. The structure also suggests a model of nucleoprotein-polymerase interaction that would enable replication and transcription solely upon local disruption of the nucleocapsid. | |||
High resolution cryo-EM structure of the helical RNA-bound Hantaan virus nucleocapsid reveals its assembly mechanisms.,Arragain B, Reguera J, Desfosses A, Gutsche I, Schoehn G, Malet H Elife. 2019 Jan 14;8. pii: 43075. doi: 10.7554/eLife.43075. PMID:30638449<ref>PMID:30638449</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6i2n" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Hantaan hantavirus]] | |||
[[Category: Large Structures]] | |||
[[Category: Spodoptera frugiperda]] | |||
[[Category: Arragain, B]] | |||
[[Category: Desfosses, A]] | |||
[[Category: Gutsche, I]] | |||
[[Category: Malet, H]] | |||
[[Category: Reguera, J]] | |||
[[Category: Schoehn, G]] | |||
[[Category: Nucleocapsid]] | |||
[[Category: Nucleoprotein]] | |||
[[Category: Rna-binding]] | |||
[[Category: Viral protein]] | |||
Latest revision as of 01:37, 11 April 2020
Helical RNA-bound Hantaan virus nucleocapsid
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