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New page: '''Unreleased structure''' The entry 6ioa is ON HOLD Authors: Xie, W., Tu, J. Description: The structure of UdgX in complex with uracil Category: Unreleased Structures [[Category: ...
 
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'''Unreleased structure'''


The entry 6ioa is ON HOLD
==The structure of UdgX in complex with uracil==
<StructureSection load='6ioa' size='340' side='right'caption='[[6ioa]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ioa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IOA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IOA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=URA:URACIL'>URA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ioa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ioa OCA], [https://pdbe.org/6ioa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ioa RCSB], [https://www.ebi.ac.uk/pdbsum/6ioa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ioa ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/I7F541_MYCS2 I7F541_MYCS2]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A uracil DNA glycosylase (UDG) from Mycobacterium smegmatis (MsmUdgX) shares sequence similarity with family 4 UDGs and forms exceedingly stable complexes with single-stranded uracil-containing DNAs (ssDNA-Us) that are resistant to denaturants. However, MsmUdgX has been reported to be inactive in excising uracil from ssDNA-Us and the underlying structural basis is unclear. Here, we report high-resolution crystal structures of MsmUdgX in the free, uracil- and DNA-bound forms, respectively. The structural information, supported by mutational and biochemical analyses, indicates that the conserved residue His109 located on a characteristic loop forms an irreversible covalent linkage with the deoxyribose at the apyrimidinic site of ssDNA-U, thus rendering the enzyme unable to regenerate. By proposing the catalytic pathway and molecular mechanism for MsmUdgX, our studies provide an insight into family 4 UDGs and UDGs in general.


Authors: Xie, W., Tu, J.
Suicide inactivation of the uracil DNA glycosylase UdgX by covalent complex formation.,Tu J, Chen R, Yang Y, Cao W, Xie W Nat Chem Biol. 2019 Jun;15(6):615-622. doi: 10.1038/s41589-019-0290-x. Epub 2019 , May 17. PMID:31101915<ref>PMID:31101915</ref>


Description: The structure of UdgX in complex with uracil
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Xie, W]]
<div class="pdbe-citations 6ioa" style="background-color:#fffaf0;"></div>
[[Category: Tu, J]]
 
==See Also==
*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycolicibacterium smegmatis MC2 155]]
[[Category: Tu J]]
[[Category: Xie W]]
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