6n0s: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "6n0s" [edit=sysop:move=sysop] |
No edit summary |
||
| (4 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==N-terminal domain of Staphylothermus marinus McrB== | |||
<StructureSection load='6n0s' size='340' side='right'caption='[[6n0s]], [[Resolution|resolution]] 1.92Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6n0s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylothermus_marinus_F1 Staphylothermus marinus F1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N0S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N0S FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n0s OCA], [https://pdbe.org/6n0s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n0s RCSB], [https://www.ebi.ac.uk/pdbsum/6n0s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n0s ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
McrBC is a conserved modification-dependent restriction system that in Escherichia coli specifically targets foreign DNA containing methylated cytosines. Crystallographic data show that the N-terminal domain of Escherichia coli McrB binds substrates via a base flipping mechanism. This region is poorly conserved among the plethora of McrB homologs, suggesting that other species may use alternative binding strategies and/or recognize different targets. Here we present the crystal structure of the N-terminal domain from Stayphlothermus marinus McrB (Sm3-180) at 1.92 A, which adopts a PUA-like EVE fold that is closely related to the YTH and ASCH RNA binding domains. Unlike most PUA-like domains, Sm3-180 binds DNA and can associate with different modified substrates. We find the canonical 'aromatic cage' binding pocket that confers specificity for methylated bases in other EVE/YTH domains is degenerate and occluded in Sm3-180, which may contribute to its promiscuity in target recognition. Further structural comparison between different PUA-like domains identifies motifs and conformational variations that correlate with the preference for binding either DNA or RNA. Together these data have important implications for PUA-like domain specificity and suggest a broader biological versatility for the McrBC family than previously described. | |||
The N-terminal domain of Staphylothermus marinus McrB shares structural homology with PUA-like RNA binding proteins.,Hosford CJ, Adams MC, Niu Y, Chappie JS J Struct Biol. 2020 Sep 1;211(3):107572. doi: 10.1016/j.jsb.2020.107572. Epub, 2020 Jul 8. PMID:32652237<ref>PMID:32652237</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6n0s" style="background-color:#fffaf0;"></div> | ||
[[Category: Hosford | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Staphylothermus marinus F1]] | |||
[[Category: Chappie JS]] | |||
[[Category: Hosford CJ]] | |||
[[Category: Niu Y]] | |||
Latest revision as of 08:09, 17 October 2024
N-terminal domain of Staphylothermus marinus McrB
| ||||||||||||
