6i97: Difference between revisions
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New page: '''Unreleased structure''' The entry 6i97 is ON HOLD Authors: Josts, I., Tidow, H. Description: Crystal structure of outer membrane protein Category: Unreleased Structures [[Catego... |
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The | ==Structure of the ferrioxamine B transporter FoxA from Pseudomonas aeruginosa in complex with ferrioxamine B and a C-terminal TonB fragment== | ||
<StructureSection load='6i97' size='340' side='right'caption='[[6i97]], [[Resolution|resolution]] 3.35Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6i97]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I97 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0UE:FERRIOXAMINE+B'>0UE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i97 OCA], [https://pdbe.org/6i97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i97 RCSB], [https://www.ebi.ac.uk/pdbsum/6i97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i97 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9I116_PSEAE Q9I116_PSEAE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Many microbes and fungi acquire the essential ion Fe(3+) through the synthesis and secretion of high-affinity chelators termed siderophores. In Gram-negative bacteria, these ferric-siderophore complexes are actively taken up using highly specific TonB-dependent transporters (TBDTs) located in the outer bacterial membrane (OM). However, the detailed mechanism of how the inner-membrane protein TonB connects to the transporters in the OM as well as the interplay between siderophore- and TonB-binding to the transporter is still poorly understood. Here, we present three crystal structures of the TBDT FoxA from Pseudomonas aeruginosa (containing a signalling domain) in complex with the siderophore ferrioxamine B and TonB and combine them with a detailed analysis of binding constants. The structures show that both siderophore and TonB-binding is required to form a translocation-competent state of the FoxA transporter in a two-step TonB-binding mechanism. The complex structure also indicates how TonB-binding influences the orientation of the signalling domain. | |||
Ternary structure of the outer membrane transporter FoxA with resolved signalling domain provides insights into TonB-mediated siderophore uptake.,Josts I, Veith K, Tidow H Elife. 2019 Aug 6;8. pii: 48528. doi: 10.7554/eLife.48528. PMID:31385808<ref>PMID:31385808</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Josts | <div class="pdbe-citations 6i97" style="background-color:#fffaf0;"></div> | ||
[[Category: Tidow | |||
==See Also== | |||
*[[TonB|TonB]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudomonas aeruginosa]] | |||
[[Category: Josts I]] | |||
[[Category: Tidow H]] | |||
