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==Crystal structure of DszA carbon methyltransferase==
==Crystal structure of DszA carbon methyltransferase==
<StructureSection load='6cca' size='340' side='right' caption='[[6cca]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
<StructureSection load='6cca' size='340' side='right'caption='[[6cca]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6cca]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CCA FirstGlance]. <br>
<table><tr><td colspan='2'>[[6cca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sorangium_cellulosum Sorangium cellulosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CCA FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cca OCA], [http://pdbe.org/6cca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cca RCSB], [http://www.ebi.ac.uk/pdbsum/6cca PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cca ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cca OCA], [https://pdbe.org/6cca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cca RCSB], [https://www.ebi.ac.uk/pdbsum/6cca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cca ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q4U447_SORCE Q4U447_SORCE]
The methyl substituents in products of trans-acyltransferase assembly lines are usually incorporated by S-adenosyl-methionine (SAM)-dependent methyltransferase (MT) domains. The gem-dimethyl moieties within the polyketide disorazol are installed through the iterative action of an MT in the third module of its assembly line. The 1.75-A-resolution crystal structure of this MT helps elucidate how it catalyzes the addition of two methyl groups. Activity assays of point mutants on beta-ketoacyl chains linked to an acyl carrier protein and N-acetylcysteamine provide additional insights into the roles of active site residues. The replacement of an alanine with a phenylalanine at an apparent gatekeeping position resulted in more monomethylation than dimethylation. MTs may form an interface with ketoreductases (KRs) and even mediate the docking of trans-acyltransferase assembly line polypeptides through this association.
 
Structural and Functional Studies of a gem-Dimethylating Methyltransferase from a trans-Acyltransferase Assembly Line.,Meinke JL, Mehaffey MR, Wagner DT, Sun N, Zhang Z, Brodbelt JS, Keatinge-Clay AT ACS Chem Biol. 2018 Nov 9. doi: 10.1021/acschembio.8b00733. PMID:30371052<ref>PMID:30371052</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6cca" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Keatinge-Clay, A T]]
[[Category: Large Structures]]
[[Category: Meinke, J L]]
[[Category: Sorangium cellulosum]]
[[Category: Disorazol]]
[[Category: Keatinge-Clay AT]]
[[Category: Gem-dimethyl]]
[[Category: Meinke JL]]
[[Category: Methyltransferase]]
[[Category: Polyketide assembly line]]
[[Category: Transferase]]

Latest revision as of 14:27, 13 March 2024

Crystal structure of DszA carbon methyltransferase

6cca, resolution 1.75Å

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