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| [[Image:378d.gif|left|200px]]
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| {{Structure
| | ==STRUCTURE OF THE SIDE-BY-SIDE BINDING OF DISTAMYCIN TO DNA== |
| |PDB= 378d |SIZE=350|CAPTION= <scene name='initialview01'>378d</scene>, resolution 2.400Å
| | <StructureSection load='378d' size='340' side='right'caption='[[378d]], [[Resolution|resolution]] 2.40Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DMY:DISTAMYCIN+A'>DMY</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
| | <table><tr><td colspan='2'>[[378d]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=378D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=378D FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| |GENE=
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMY:DISTAMYCIN+A'>DMY</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=378d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=378d OCA], [https://pdbe.org/378d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=378d RCSB], [https://www.ebi.ac.uk/pdbsum/378d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=378d ProSAT]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=378d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=378d OCA], [http://www.ebi.ac.uk/pdbsum/378d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=378d RCSB]</span>
| | __TOC__ |
| }}
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| '''STRUCTURE OF THE SIDE-BY-SIDE BINDING OF DISTAMYCIN TO DNA'''
| | [[Category: Mitra SN]] |
| | | [[Category: Sundaralingam M]] |
| | | [[Category: Wahl MC]] |
| ==Overview==
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| The 2.40 A resolution crystal structure of a side-by-side binding of distamycin A molecules to a DNA octamer d(GTATATAC)2 with an extended alternating TA sequence has been determined. The unit-cell parameters are a = 29.55, b = 42.18, c = 43.38 A, beta = 96.56 degrees, space group P21, with two molecules in the asymmetric unit, in contrast to all previous side-by-side distamycin-DNA complexes which have only a single DNA strand and one drug molecule in the asymmetric unit. The structure was solved by the molecular-replacement method and refined to an R index of 21.0% using 3467 reflections [>/= 2sigma(F)]. The minor grooves of the DNA molecules bind two side-by-side antiparallel staggered distamycins spanning about five base pairs and virtually covering the entire length of the DNA. The octamer duplexes exhibit low-high alternations in the helical twist, sugar puckering and the C-O3' and O3'-P torsion angles, similar to the earlier side-by-side complexes containing inosine bases. The molecules are stacked one over the other along the ac diagonal in an infinite pseudo-continuous helical column with no lateral interactions.
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| ==About this Structure==
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| 378D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=378D OCA].
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| ==Reference==
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| Structure of the side-by-side binding of distamycin to d(GTATATAC)2., Mitra SN, Wahl MC, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):602-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089456 10089456]
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| [[Category: Protein complex]] | |
| [[Category: Mitra, S N.]] | |
| [[Category: Sundaralingam, M.]] | |
| [[Category: Wahl, M C.]] | |
| [[Category: double drug in minor groove]]
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| [[Category: double helix]]
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| [[Category: right handed dna]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:21:56 2008''
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