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| [[Image:398d.gif|left|200px]]
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| {{Structure
| | ==3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION== |
| |PDB= 398d |SIZE=350|CAPTION= <scene name='initialview01'>398d</scene>, resolution 1.940Å
| | <StructureSection load='398d' size='340' side='right'caption='[[398d]], [[Resolution|resolution]] 1.94Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| | <table><tr><td colspan='2'>[[398d]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=398D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=398D FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94Å</td></tr> |
| |GENE=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=398d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=398d OCA], [https://pdbe.org/398d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=398d RCSB], [https://www.ebi.ac.uk/pdbsum/398d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=398d ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=
| | __TOC__ |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=398d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=398d OCA], [http://www.ebi.ac.uk/pdbsum/398d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=398d RCSB]</span>
| | </StructureSection> |
| }}
| | [[Category: Large Structures]] |
| | | [[Category: Cellai L]] |
| '''3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION'''
| | [[Category: Heumann H]] |
| | | [[Category: Meier G]] |
| | | [[Category: Mochi-Onori A]] |
| ==Overview==
| | [[Category: Mueller U]] |
| During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A resolution of an eight-base pair hybrid duplex representing the junction to identify global or local structural perturbations which may be recognized by HIV-RT RNase H. The junction octamer is in a global A-type conformation throughout. A base pair step with distinct stacking geometry and variable backbone conformation is located next to the main endonucleolytic cleavage site. This base pair step may serve as a recognition site for HIV-RT RNase H.
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| ==About this Structure==
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| 398D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=398D OCA].
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| ==Reference==
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| Crystal structure of an eight-base pair duplex containing the 3'-DNA-RNA-5' junction formed during initiation of minus-strand synthesis of HIV replication., Mueller U, Maier G, Mochi Onori A, Cellai L, Heumann H, Heinemann U, Biochemistry. 1998 Sep 1;37(35):12005-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9724510 9724510]
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| [[Category: Protein complex]] | |
| [[Category: Cellai, L.]] | |
| [[Category: Heumann, H.]] | |
| [[Category: Meier, G.]] | |
| [[Category: Mochi-Onori, A.]] | |
| [[Category: Mueller, U.]] | |
| [[Category: a-dna/rna]]
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| [[Category: double helix]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:22:09 2008''
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