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==Crystal structure of ST1710 complexed with its promoter DNA==
==Crystal structure of ST1710 complexed with its promoter DNA==
<StructureSection load='3gfi' size='340' side='right' caption='[[3gfi]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
<StructureSection load='3gfi' size='340' side='right'caption='[[3gfi]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3gfi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_16993 Dsm 16993]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GFI FirstGlance]. <br>
<table><tr><td colspan='2'>[[3gfi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii Sulfurisphaera tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GFI FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2eb7|2eb7]], [[2yr2|2yr2]], [[3gez|3gez]], [[3gf2|3gf2]], [[3gfj|3gfj]], [[3gfl|3gfl]], [[3gfm|3gfm]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ST1710 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 DSM 16993])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gfi OCA], [https://pdbe.org/3gfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gfi RCSB], [https://www.ebi.ac.uk/pdbsum/3gfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gfi ProSAT], [https://www.topsan.org/Proteins/RSGI/3gfi TOPSAN]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gfi OCA], [http://pdbe.org/3gfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gfi RCSB], [http://www.ebi.ac.uk/pdbsum/3gfi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gfi ProSAT], [http://www.topsan.org/Proteins/RSGI/3gfi TOPSAN]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q96ZY1_SULTO Q96ZY1_SULTO]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gfi ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gfi ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
ST1710, a member of the multiple antibiotic resistance regulator (MarR) family of regulatory proteins in bacteria and archaea, plays important roles in development of antibiotic resistance, a global health problem. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 complexed with salicylate, a well-known inhibitor of MarR proteins and the ST1710 complex with its promoter DNA, refined to 1.8 and 2.10 A resolutions, respectively. The ST1710-DNA complex shares the topology of apo-ST1710 and MarR proteins, with each subunit containing a winged helix-turn-helix (wHtH) DNA binding motif. Significantly large conformational changes occurred upon DNA binding and in each of the dimeric monomers in the asymmetric unit of the ST1710-DNA complex. Conserved wHtH loop residues interacting with the bound DNA and mutagenic analysis indicated that R89, R90 and K91 were important for DNA recognition. Significantly, the bound DNA exhibited a new binding mechanism.
ST1710-DNA complex crystal structure reveals the DNA binding mechanism of the MarR family of regulators.,Kumarevel T, Tanaka T, Umehara T, Yokoyama S Nucleic Acids Res. 2009 Aug;37(14):4723-35. Epub 2009 Jun 9. PMID:19509310<ref>PMID:19509310</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3gfi" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Dsm 16993]]
[[Category: Large Structures]]
[[Category: Kumarevel, T]]
[[Category: Sulfurisphaera tokodaii]]
[[Category: Structural genomic]]
[[Category: Kumarevel T]]
[[Category: Tanaka, T]]
[[Category: Tanaka T]]
[[Category: Yokoyama, S]]
[[Category: Yokoyama S]]
[[Category: Dna-binding]]
[[Category: Marr]]
[[Category: National project on protein structural and functional analyse]]
[[Category: Nppsfa]]
[[Category: Rsgi]]
[[Category: St1710]]
[[Category: Transcription]]
[[Category: Transcription regulation]]
[[Category: Transcription regulator]]
[[Category: Transcription-dna complex]]
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