6n1c: Difference between revisions
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==Crystal structure of Inorganic pyrophosphatase from Legionella pneumophila Philadelphia 1== | ==Crystal structure of Inorganic pyrophosphatase from Legionella pneumophila Philadelphia 1== | ||
<StructureSection load='6n1c' size='340' side='right' caption='[[6n1c]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='6n1c' size='340' side='right'caption='[[6n1c]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6n1c]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1C OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6n1c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N1C FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALA:ALANINE'>ALA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALA:ALANINE'>ALA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n1c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n1c OCA], [https://pdbe.org/6n1c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n1c RCSB], [https://www.ebi.ac.uk/pdbsum/6n1c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n1c ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/Q5ZRW2_LEGPH Q5ZRW2_LEGPH] Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.[HAMAP-Rule:MF_00209] | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Inorganic pyrophosphate (PP(i)) is generated as an intermediate or byproduct of many fundamental metabolic pathways, including DNA/RNA synthesis. The intracellular concentration of PP(i) must be regulated as buildup can inhibit many critical cellular processes. Inorganic pyrophosphatases (PPases) hydrolyze PP(i) into two orthophosphates (P(i)), preventing the toxic accumulation of the PP(i) byproduct in cells and making P(i) available for use in biosynthetic pathways. Here, the crystal structure of a family I inorganic pyrophosphatase from Legionella pneumophila is reported at 2.0 A resolution. L. pneumophila PPase (LpPPase) adopts a homohexameric assembly and shares the oligonucleotide/oligosaccharide-binding (OB) beta-barrel core fold common to many other bacterial family I PPases. LpPPase demonstrated hydrolytic activity against a general substrate, with Mg(2+) being the preferred metal cofactor for catalysis. Legionnaires' disease is a severe respiratory infection caused primarily by L. pneumophila, and thus increased characterization of the L. pneumophila proteome is of interest. | |||
Characterization of a family I inorganic pyrophosphatase from Legionella pneumophila Philadelphia 1.,Moorefield J, Konuk Y, Norman JO, Abendroth J, Edwards TE, Lorimer DD, Mayclin SJ, Staker BL, Craig JK, Barett KF, Barrett LK, Van Voorhis WC, Myler PJ, McLaughlin KJ Acta Crystallogr F Struct Biol Commun. 2023 Oct 1;79(Pt 10):257-266. doi: , 10.1107/S2053230X23008002. Epub 2023 Sep 20. PMID:37728609<ref>PMID:37728609</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6n1c" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Inorganic pyrophosphatase 3D structures|Inorganic pyrophosphatase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]] | ||
Latest revision as of 12:27, 17 July 2024
Crystal structure of Inorganic pyrophosphatase from Legionella pneumophila Philadelphia 1
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