6inb

Crystal structure of an acetolactate decarboxylase from Klebsiella pneumoniae

spinqualitylabelsall models 6inb, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6inb is a 1 chain structure with sequence from Klebsiella pneumoniae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Ligands: CL, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function W9BHF3_KLEPN Publication Abstract from PubMed Acetolactate decarboxylase (ALDC) is a well-characterized anabolic enzyme involved with 3-hydroxy butanone (acetoin), an important physiological metabolite excreted by microbes. Although the enzyme is widely present in microorganisms, few atomic structures and functions of ALDC have been reported to date. Here we report the crystal structure of ALDC from Klebsiella pneumoniae KP (KpALDC). KpALDC crystallizes in space group P3121 with one monomer per asymmetric unit. Analytical ultracentrifugation data shows that KpALDC forms a stable dimer but can exist as a tetramer in solution. A Zn(2+) ion is coordinated by three strictly-conserved histidines (His198, His200 and His211) and a conserved glutamate (Glu69), but the C-terminal tail that forms part of the active site in ALDC enzymes is disordered. A complex structure with ethane-1,2-diol shows a unusual mode of binding, whereby the ligand does not coordinate the Zn(2+) ion. MicroScale Thermophoresis analysis shows that KpALDC binds Zn(2+) ions, but no binding of Mg(2+), Ca(2+) and Mn(2+) ions was detected. Structural characterization of an acetolactate decarboxylase from Klebsiella pneumoniae.,Wu W, Zhao Q, Che S, Jia H, Liang H, Zhang H, Liu R, Zhang Q, Bartlam M Biochem Biophys Res Commun. 2019 Jan 29;509(1):154-160. doi:, 10.1016/j.bbrc.2018.12.094. Epub 2018 Dec 21. PMID:30580999 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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