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==LasR LBD L130F:3OC10HSL complex==
==LasR LBD L130F:3OC10HSL complex==
<StructureSection load='6mvn' size='340' side='right' caption='[[6mvn]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='6mvn' size='340' side='right'caption='[[6mvn]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6mvn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MVN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MVN FirstGlance]. <br>
<table><tr><td colspan='2'>[[6mvn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MVN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MVN FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3M5:3-OXO-N-[(3S)-2-OXOTETRAHYDROFURAN-3-YL]DECANAMIDE'>3M5</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mvn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mvn OCA], [http://pdbe.org/6mvn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mvn RCSB], [http://www.ebi.ac.uk/pdbsum/6mvn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mvn ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3M5:3-OXO-N-[(3S)-2-OXOTETRAHYDROFURAN-3-YL]DECANAMIDE'>3M5</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mvn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mvn OCA], [https://pdbe.org/6mvn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mvn RCSB], [https://www.ebi.ac.uk/pdbsum/6mvn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mvn ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A0A0H2Z901_PSEAB A0A0H2Z901_PSEAB]
Quorum sensing is a cell-cell communication process that bacteria use to orchestrate group behaviors. Quorum sensing is mediated by signal molecules called autoinducers. Autoinducers are often structurally similar, raising questions concerning how bacteria distinguish among them. Here, we use the Pseudomonas aeruginosa LasR quorum-sensing receptor to explore signal discrimination. The cognate autoinducer, 3OC12 homoserine lactone (3OC12HSL), is a more potent activator of LasR than other homoserine lactones. However, other homoserine lactones can elicit LasR-dependent quorum-sensing responses, showing that LasR displays ligand promiscuity. We identify mutants that alter which homoserine lactones LasR detects. Substitution at residue S129 decreases the LasR response to 3OC12HSL, while enhancing discrimination against noncognate autoinducers. Conversely, the LasR L130F mutation increases the potency of 3OC12HSL and other homoserine lactones. We solve crystal structures of LasR ligand-binding domains complexed with noncognate autoinducers. Comparison with existing structures reveals that ligand selectivity/sensitivity is mediated by a flexible loop near the ligand-binding site. We show that LasR variants with modified ligand preferences exhibit altered quorum-sensing responses to autoinducers in vivo. We suggest that possessing some ligand promiscuity endows LasR with the ability to optimally regulate quorum-sensing traits.


Structural determinants driving homoserine lactone ligand selection in the Pseudomonas aeruginosa LasR quorum-sensing receptor.,McCready AR, Paczkowski JE, Henke BR, Bassler BL Proc Natl Acad Sci U S A. 2019 Jan 2;116(1):245-254. doi:, 10.1073/pnas.1817239116. Epub 2018 Dec 17. PMID:30559209<ref>PMID:30559209</ref>
==See Also==
 
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6mvn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bassler, B L]]
[[Category: Large Structures]]
[[Category: Paczkowski, J E]]
[[Category: Pseudomonas aeruginosa UCBPP-PA14]]
[[Category: Transcription]]
[[Category: Bassler BL]]
[[Category: Transcriptional activator]]
[[Category: Paczkowski JE]]
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