6j6k: Difference between revisions
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New page: '''Unreleased structure''' The entry 6j6k is ON HOLD Authors: Fan, X., Wang, J., Lei, J.L., Wang, H.W. Description: apo-state streptavidin Category: Unreleased Structures [[Categor... |
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The | ==Apo-state streptavidin== | ||
<SX load='6j6k' size='340' side='right' viewer='molstar' caption='[[6j6k]], [[Resolution|resolution]] 3.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6j6k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J6K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J6K FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j6k OCA], [https://pdbe.org/6j6k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j6k RCSB], [https://www.ebi.ac.uk/pdbsum/6j6k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j6k ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular weight higher than 300 kDa at ~3 A resolution. However, it remains a challenge to obtain the high-resolution structures of molecules smaller than 200 kDa using single-particle cryo-EM. In this work, we apply the Cs-corrector-VPP-coupled cryo-EM to study the 52 kDa streptavidin (SA) protein supported on a thin layer of graphene and embedded in vitreous ice. We are able to solve both the apo-SA and biotin-bound SA structures at near-atomic resolution using single-particle cryo-EM. We demonstrate that the method has the potential to determine the structures of molecules as small as 39 kDa. | |||
Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution.,Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J, Wang HW Nat Commun. 2019 Jun 3;10(1):2386. doi: 10.1038/s41467-019-10368-w. PMID:31160591<ref>PMID:31160591</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6j6k" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: Wang | *[[Avidin 3D structures|Avidin 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Large Structures]] | |||
[[Category: Streptomyces avidinii]] | |||
[[Category: Fan X]] | |||
[[Category: Lei JL]] | |||
[[Category: Wang HW]] | |||
[[Category: Wang J]] | |||
Latest revision as of 05:46, 3 July 2025
Apo-state streptavidin
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