6j7a: Difference between revisions

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New page: '''Unreleased structure''' The entry 6j7a is ON HOLD Authors: Sugishima, M., Sato, H., Wada, K., Yamamoto, K. Description: Fusion protein of heme oxygenase-1 and NADPH cytochrome P450 ...
 
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'''Unreleased structure'''


The entry 6j7a is ON HOLD
==Fusion protein of heme oxygenase-1 and NADPH cytochrome P450 reductase (17aa)==
<StructureSection load='6j7a' size='340' side='right'caption='[[6j7a]], [[Resolution|resolution]] 3.27&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6j7a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J7A FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.269&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j7a OCA], [https://pdbe.org/6j7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j7a RCSB], [https://www.ebi.ac.uk/pdbsum/6j7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j7a ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HMOX1_RAT HMOX1_RAT] Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.[https://www.uniprot.org/uniprot/NCPR_RAT NCPR_RAT] This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Heme oxygenase-1 (HMOX1) catalyzes heme degradation utilizing reducing equivalents supplied from NADPH-cytochrome P450 reductase (CYPOR). Recently, we determined the complex structure of NADP(+) -bound open-conformation stabilized CYPOR and heme-HMOX1, but the resolution was limited to 4.3 A. Here, we determined the crystal structure of the fusion protein of open-conformation stabilized CYPOR and heme-HMOX1 at 3.25 A resolution. Unexpectedly, no NADP(+) was bound to this fusion protein in the crystal. Structural comparison of the NADP(+) -bound complex and the NADP(+) -free fusion protein suggests that NADP(+) binding regulates the conformational change in the FAD-binding domain of CYPOR. As a result of this change, the FMN-binding domain of CYPOR approaches heme-bound HMOX1 upon NADP(+) binding to enhance the electron-transfer efficiency from FMN to heme.


Authors: Sugishima, M., Sato, H., Wada, K., Yamamoto, K.
Crystal structure of a NADPH-cytochrome P450 oxidoreductase (CYPOR) and heme oxygenase 1 fusion protein implies a conformational change in CYPOR upon NADPH/NADP(+) binding.,Sugishima M, Sato H, Wada K, Yamamoto K FEBS Lett. 2019 Mar 18. doi: 10.1002/1873-3468.13360. PMID:30883732<ref>PMID:30883732</ref>


Description: Fusion protein of heme oxygenase-1 and NADPH cytochrome P450 reductase (17aa)
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Yamamoto, K]]
<div class="pdbe-citations 6j7a" style="background-color:#fffaf0;"></div>
[[Category: Wada, K]]
== References ==
[[Category: Sugishima, M]]
<references/>
[[Category: Sato, H]]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Sato H]]
[[Category: Sugishima M]]
[[Category: Wada K]]
[[Category: Yamamoto K]]
Retrieved from "https://proteopedia.org/6j7a"