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==Cryo-EM structure of the Ceru+32/GFP-17 protomer==
==Cryo-EM structure of the Ceru+32/GFP-17 protomer==
<StructureSection load='6mdr' size='340' side='right' caption='[[6mdr]], [[Resolution|resolution]] 3.47&Aring;' scene=''>
<SX load='6mdr' size='340' side='right' viewer='molstar' caption='[[6mdr]], [[Resolution|resolution]] 3.47&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6mdr]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MDR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MDR FirstGlance]. <br>
<table><tr><td colspan='2'>[[6mdr]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MDR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MDR FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.47&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mdr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mdr OCA], [http://pdbe.org/6mdr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mdr RCSB], [http://www.ebi.ac.uk/pdbsum/6mdr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mdr ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mdr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mdr OCA], [https://pdbe.org/6mdr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mdr RCSB], [https://www.ebi.ac.uk/pdbsum/6mdr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mdr ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Symmetrical protein oligomers are ubiquitous in biological systems and perform key structural and regulatory functions. However, there are few methods for constructing such oligomers. Here we have engineered completely synthetic, symmetrical oligomers by combining pairs of oppositely supercharged variants of a normally monomeric model protein through a strategy we term 'supercharged protein assembly' (SuPrA). We show that supercharged variants of green fluorescent protein can assemble into a variety of architectures including a well-defined symmetrical 16-mer structure that we solved using cryo-electron microscopy at 3.47 A resolution. The 16-mer is composed of two stacked rings of octamers, in which the octamers contain supercharged proteins of alternating charges, and interactions within and between the rings are mediated by a variety of specific electrostatic contacts. The ready assembly of this structure suggests that combining oppositely supercharged pairs of protein variants may provide broad opportunities for generating novel architectures via otherwise unprogrammed interactions.


Supercharging enables organized assembly of synthetic biomolecules.,Simon AJ, Zhou Y, Ramasubramani V, Glaser J, Pothukuchy A, Gollihar J, Gerberich JC, Leggere JC, Morrow BR, Jung C, Glotzer SC, Taylor DW, Ellington AD Nat Chem. 2019 Jan 14. pii: 10.1038/s41557-018-0196-3. doi:, 10.1038/s41557-018-0196-3. PMID:30643229<ref>PMID:30643229</ref>
==See Also==
 
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6mdr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Aeqvi]]
[[Category: Aequorea victoria]]
[[Category: Ellington, A D]]
[[Category: Large Structures]]
[[Category: Gerberich, J C]]
[[Category: Ellington AD]]
[[Category: Glaser, J]]
[[Category: Gerberich JC]]
[[Category: Glotzer, S C]]
[[Category: Glaser J]]
[[Category: Golihar, J]]
[[Category: Glotzer SC]]
[[Category: Jung, C]]
[[Category: Golihar J]]
[[Category: Leggere, J C]]
[[Category: Jung C]]
[[Category: Morrow, B R]]
[[Category: Leggere JC]]
[[Category: Pothukuchy, A]]
[[Category: Morrow BR]]
[[Category: Ramasubramani, V]]
[[Category: Pothukuchy A]]
[[Category: Simon, A J]]
[[Category: Ramasubramani V]]
[[Category: Taylor, D W]]
[[Category: Simon AJ]]
[[Category: Zhou, Y]]
[[Category: Taylor DW]]
[[Category: 16-mer]]
[[Category: Zhou Y]]
[[Category: D4 symmetry]]
[[Category: Electrostatic interaction]]
[[Category: Luminescent protein]]
[[Category: Supercharged protein assembly]]

Latest revision as of 14:42, 13 March 2024

Cryo-EM structure of the Ceru+32/GFP-17 protomer

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