6c3i: Difference between revisions

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==Crystal structure of the Deinococcus radiodurans Nramp/MntH divalent transition metal transporter G45R mutant in an inward occluded state==
==Crystal structure of the Deinococcus radiodurans Nramp/MntH divalent transition metal transporter G45R mutant in an inward occluded state==
<StructureSection load='6c3i' size='340' side='right' caption='[[6c3i]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
<StructureSection load='6c3i' size='340' side='right'caption='[[6c3i]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6c3i]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C3I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C3I FirstGlance]. <br>
<table><tr><td colspan='2'>[[6c3i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans_R1 Deinococcus radiodurans R1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C3I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6C3I FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9501262&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c3i OCA], [http://pdbe.org/6c3i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c3i RCSB], [http://www.ebi.ac.uk/pdbsum/6c3i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c3i ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6c3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c3i OCA], [https://pdbe.org/6c3i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6c3i RCSB], [https://www.ebi.ac.uk/pdbsum/6c3i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6c3i ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/MNTH_DEIRA MNTH_DEIRA]] H(+)-stimulated, divalent metal cation uptake system.  
[https://www.uniprot.org/uniprot/MNTH_DEIRA MNTH_DEIRA] H(+)-stimulated, divalent metal cation uptake system.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Nramp family transporters-expressed in organisms from bacteria to humans-enable uptake of essential divalent transition metals via an alternating-access mechanism that also involves proton transport. We present high-resolution structures of Deinococcus radiodurans (Dra)Nramp in multiple conformations to provide a thorough description of the Nramp transport cycle by identifying the key intramolecular rearrangements and changes to the metal coordination sphere. Strikingly, while metal transport requires cycling from outward- to inward-open states, efficient proton transport still occurs in outward-locked (but not inward-locked) DraNramp. We propose a model in which metal and proton enter the transporter via the same external pathway to the binding site, but follow separate routes to the cytoplasm, which could facilitate the co-transport of two cationic species. Our results illustrate the flexibility of the LeuT fold to support a broad range of substrate transport and conformational change mechanisms.
 
Structures in multiple conformations reveal distinct transition metal and proton pathways in an Nramp transporter.,Bozzi AT, Zimanyi CM, Nicoludis JM, Lee BK, Zhang CH, Gaudet R Elife. 2019 Feb 4;8. pii: 41124. doi: 10.7554/eLife.41124. PMID:30714568<ref>PMID:30714568</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6c3i" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bozzi, A T]]
[[Category: Deinococcus radiodurans R1]]
[[Category: Gaudet, R]]
[[Category: Large Structures]]
[[Category: Zimanyi, C M]]
[[Category: Bozzi AT]]
[[Category: Divalent transition metal transporter]]
[[Category: Gaudet R]]
[[Category: Leut-fold]]
[[Category: Zimanyi CM]]
[[Category: Manganese importer]]
[[Category: Proton-coupled secondary transporter]]
[[Category: Transport protein]]
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