Dynamin: Difference between revisions

Latest revision as of 09:46, 20 February 2019

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Human dynamin 1 GTPase+GTPase effector domains complex with GDP and ethylene glycol (PDB code 5d3q)

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Function

Dynamin (DNM) is a GTPase which mediates endocytosis in membrane scission^[1] and organelle division. Dynamin 1-like mediates outer mitochondrial membrane fission^[2]. DNM is a multidomain GTPase. Mammalian genomes contain 3 DNM genes which show ca. 80% homology. DNM 1 is localized to neurons, DNM 2 is expressed ubiquitously and DNM 3 is found predominantly in the brain^[3]. The DNM 1 domain structure includes GTPase, middle, Plextrin homology, GTPase effector and proline- and arginine-rich (PRD) domains.

Disease

Mutations in DNM-2 cause centronuclear myopathy^[4].

Structural highlights

The GTPase domain of DNM 1 contains several nucleotide binding motifs which interact with the bound GDP. Motif GGQSAGKS residues 38-45; Motif TKLD residues 205-208; Motif NRSQKDIDGKK reidues 236-246^[5]. Water molecules are shown as red spheres. Whole binding site.

Dynamin 3D structures

See Dynamin 3D structures

↑ Taylor MJ, Lampe M, Merrifield CJ. A feedback loop between dynamin and actin recruitment during clathrin-mediated endocytosis. PLoS Biol. 2012;10(4):e1001302. doi: 10.1371/journal.pbio.1001302. Epub 2012 Apr , 10. PMID:22505844 doi:https://dx.doi.org/10.1371/journal.pbio.1001302
↑ Frank S, Gaume B, Bergmann-Leitner ES, Leitner WW, Robert EG, Catez F, Smith CL, Youle RJ. The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis. Dev Cell. 2001 Oct;1(4):515-25. PMID:11703942
↑ Ferguson SM, De Camilli P. Dynamin, a membrane-remodelling GTPase. Nat Rev Mol Cell Biol. 2012 Jan 11;13(2):75-88. doi: 10.1038/nrm3266. PMID:22233676 doi:https://dx.doi.org/10.1038/nrm3266
↑ Bitoun M, Maugenre S, Jeannet PY, Lacene E, Ferrer X, Laforet P, Martin JJ, Laporte J, Lochmuller H, Beggs AH, Fardeau M, Eymard B, Romero NB, Guicheney P. Mutations in dynamin 2 cause dominant centronuclear myopathy. Nat Genet. 2005 Nov;37(11):1207-9. Epub 2005 Oct 16. PMID:16227997 doi:ng1657
↑ Anand R, Eschenburg S, Reubold TF. Crystal structure of the GTPase domain and the bundle signalling element of dynamin in the GDP state. Biochem Biophys Res Commun. 2016 Jan 1;469(1):76-80. doi:, 10.1016/j.bbrc.2015.11.074. Epub 2015 Nov 21. PMID:26612256 doi:https://dx.doi.org/10.1016/j.bbrc.2015.11.074

References

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

[1] Taylor MJ, Lampe M, Merrifield CJ. A feedback loop between dynamin and actin recruitment during clathrin-mediated endocytosis. PLoS Biol. 2012;10(4):e1001302. doi: 10.1371/journal.pbio.1001302. Epub 2012 Apr , 10. PMID:22505844 doi:https://dx.doi.org/10.1371/journal.pbio.1001302

[2] Frank S, Gaume B, Bergmann-Leitner ES, Leitner WW, Robert EG, Catez F, Smith CL, Youle RJ. The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis. Dev Cell. 2001 Oct;1(4):515-25. PMID:11703942

[3] Ferguson SM, De Camilli P. Dynamin, a membrane-remodelling GTPase. Nat Rev Mol Cell Biol. 2012 Jan 11;13(2):75-88. doi: 10.1038/nrm3266. PMID:22233676 doi:https://dx.doi.org/10.1038/nrm3266

[4] Bitoun M, Maugenre S, Jeannet PY, Lacene E, Ferrer X, Laforet P, Martin JJ, Laporte J, Lochmuller H, Beggs AH, Fardeau M, Eymard B, Romero NB, Guicheney P. Mutations in dynamin 2 cause dominant centronuclear myopathy. Nat Genet. 2005 Nov;37(11):1207-9. Epub 2005 Oct 16. PMID:16227997 doi:ng1657

[5] Anand R, Eschenburg S, Reubold TF. Crystal structure of the GTPase domain and the bundle signalling element of dynamin in the GDP state. Biochem Biophys Res Commun. 2016 Jan 1;469(1):76-80. doi:, 10.1016/j.bbrc.2015.11.074. Epub 2015 Nov 21. PMID:26612256 doi:https://dx.doi.org/10.1016/j.bbrc.2015.11.074

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-<StructureSection load='5d3q' size='340' side='right' caption='Human dynamin 1 GTPase+GTPase effector domains complex with GDP and ethylene glycol (PDB code [[5d3q]])' scene=''>
+<StructureSection load='5d3q' size='400' side='right' caption='Human dynamin 1 GTPase+GTPase effector domains complex with GDP and ethylene glycol (PDB code [[5d3q]])' scene='80/808513/Cv/1'>
 == Function ==
@@ Line 10: / Line 10: @@
 == Structural highlights ==
+The GTPase domain of DNM 1 contains several nucleotide binding motifs which interact with the bound GDP. <scene name='80/808513/Cv/6'>Motif GGQSAGKS</scene> residues 38-45; <scene name='80/808513/Cv/7'>Motif TKLD</scene> residues 205-208; <scene name='80/808513/Cv/8'>Motif NRSQKDIDGKK</scene> reidues 236-246<ref>PMID:26612256</ref>. Water molecules are shown as red spheres. <scene name='80/808513/Cv/9'>Whole binding site</scene>.
 ==Dynamin 3D structures==

Dynamin: Difference between revisions

Latest revision as of 09:46, 20 February 2019

Contents

Function

Disease

Structural highlights

Dynamin 3D structures

References

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