6jic: Difference between revisions
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New page: '''Unreleased structure''' The entry 6jic is ON HOLD Authors: Tan, W.L., Wong, K.H., Huang, J.Y., Tay, S.V., Wang, S.J. Description: Identification and Characterization of a carboxypep... |
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==Identification and Characterization of a carboxypeptidase inhibitor from Lycium barbarum== | |||
<StructureSection load='6jic' size='340' side='right'caption='[[6jic]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6jic]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lycium_barbarum Lycium barbarum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JIC FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jic OCA], [https://pdbe.org/6jic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jic RCSB], [https://www.ebi.ac.uk/pdbsum/6jic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jic ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Hyperstable cysteine-rich peptides (CRPs) represent an underexplored superfamily of bioactives in functional foods. An example is wolfberry of the Lycium barbarum family. Previously, we discovered a CRP, designated alpha-lybatide, from L. barbarum bark. Herein, we report the discovery of beta-lybatide, a novel carboxypeptidase inhibitor belonging to a different CRP family from the wolfberry plant. Proteomic and transcriptomic analyses showed that beta-lybatide contains 36 amino acids with six cysteine residues. NMR spectroscopy revealed that beta-lybatide displays a knottin-like structure that renders it highly resistant to thermal, chemical and enzymatic degradation, conditions important for keeping its structural integrity in gastrointestinal tract. Biochemical assays showed that beta-lybatide is a potent carboxypeptidase inhibitor which could contribute to the wolfberry biological activities. Bioinformatics analysis revealed an additional 49 beta-lybatide-like plant carboxypeptidase inhibitors. Together, our results show that beta-lybatide is the first and the smallest plant-derived hyperstable carboxypeptidase inhibitor discovered from a functional food. | |||
Identification and characterization of a wolfberry carboxypeptidase inhibitor from Lycium barbarum.,Huang J, Wong KH, Tan WL, Tay SV, Wang S, Tam JP Food Chem. 2021 Feb 19;351:129338. doi: 10.1016/j.foodchem.2021.129338. PMID:33647700<ref>PMID:33647700</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6jic" style="background-color:#fffaf0;"></div> | ||
[[Category: Tan | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: Wong | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Lycium barbarum]] | |||
[[Category: Huang JY]] | |||
[[Category: Tan WL]] | |||
[[Category: Tay SV]] | |||
[[Category: Wang SJ]] | |||
[[Category: Wong KH]] | |||
