6qsr: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: '''Unreleased structure''' The entry 6qsr is ON HOLD Authors: Grammbitter, G.L.C., Schmalhofer, M., Groll, M., Bode, H. Description: The Dehydratase Heterocomplex ApeI:P from Xenorhabd... |
No edit summary |
||
| (4 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==The Dehydratase Heterocomplex ApeI:P from Xenorhabdus doucetiae== | ||
<StructureSection load='6qsr' size='340' side='right'caption='[[6qsr]], [[Resolution|resolution]] 1.85Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6qsr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenorhabdus_doucetiae Xenorhabdus doucetiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QSR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QSR FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qsr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qsr OCA], [https://pdbe.org/6qsr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qsr RCSB], [https://www.ebi.ac.uk/pdbsum/6qsr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qsr ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A068QWX2_9GAMM A0A068QWX2_9GAMM] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Aryl polyene (APE) pigments are a widely distributed class of bacterial polyketides. So far, little is known about the biosynthesis of these compounds, which are produced by a novel type II polyketide synthase (PKS). We have identi-fied all enzymes involved in APE biosynthesis and determined their peculiar functions. The biosynthesis was recon-stituted in vitro and ACP-bound intermediates were assigned for each reaction step by HPLC-MS. Native mass spec-trometry experiments identified four stable complexes: the acyl-carrier proteins ApeE and ApeF bound to the thi-oesterase ApeK, the dehydratases ApeI and ApeP as well as the ketosynthase ApeO in complex with its chain-length factor ApeC. X-ray structures of the heterodimeric ApeO:ApeC and ApeI:ApeP complexes depict striking protein-protein interactions. Altogether, our study elucidated mechanistic aspects of APE biosynthesis that unifies elements of type II fatty acid and PKS systems, but in addition includes novel enzyme complexes. | |||
An Uncommon Type II PKS Catalyzes Biosynthesis of Aryl Polyene Pigments.,Grammbitter GLC, Schmalhofer M, Karimi K, Schoner TA, Tobias NJ, Morgner N, Groll M, Bode HB J Am Chem Soc. 2019 Mar 25. doi: 10.1021/jacs.8b10776. PMID:30908039<ref>PMID:30908039</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Bode | <div class="pdbe-citations 6qsr" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Beta-hydroxyacyl-acyl carrier protein dehydratase 3D structures|Beta-hydroxyacyl-acyl carrier protein dehydratase 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Xenorhabdus doucetiae]] | |||
[[Category: Bode H]] | |||
[[Category: Grammbitter GLC]] | |||
[[Category: Groll M]] | |||
[[Category: Schmalhofer M]] | |||
Latest revision as of 12:07, 24 January 2024
The Dehydratase Heterocomplex ApeI:P from Xenorhabdus doucetiae
| ||||||||||||
