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==CasX-gRNA-DNA(30bp) State II==
==CasX-gRNA-DNA(30bp) State II==
<StructureSection load='6ny1' size='340' side='right' caption='[[6ny1]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
<SX load='6ny1' size='340' side='right' viewer='molstar' caption='[[6ny1]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6ny1]] is a 4 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6e7a 6e7a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NY1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NY1 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6ny1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Deltaproteobacteria_bacterium Deltaproteobacteria bacterium]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6e7a 6e7a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NY1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NY1 FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ny1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ny1 OCA], [http://pdbe.org/6ny1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ny1 RCSB], [http://www.ebi.ac.uk/pdbsum/6ny1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ny1 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ny1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ny1 OCA], [https://pdbe.org/6ny1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ny1 RCSB], [https://www.ebi.ac.uk/pdbsum/6ny1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ny1 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A0A357BT59_9DELT A0A357BT59_9DELT]
The RNA-guided CRISPR-associated (Cas) proteins Cas9 and Cas12a provide adaptive immunity against invading nucleic acids, and function as powerful tools for genome editing in a wide range of organisms. Here we reveal the underlying mechanisms of a third, fundamentally distinct RNA-guided genome-editing platform named CRISPR-CasX, which uses unique structures for programmable double-stranded DNA binding and cleavage. Biochemical and in vivo data demonstrate that CasX is active for Escherichia coli and human genome modification. Eight cryo-electron microscopy structures of CasX in different states of assembly with its guide RNA and double-stranded DNA substrates reveal an extensive RNA scaffold and a domain required for DNA unwinding. These data demonstrate how CasX activity arose through convergent evolution to establish an enzyme family that is functionally separate from both Cas9 and Cas12a.
 
CasX enzymes comprise a distinct family of RNA-guided genome editors.,Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA Nature. 2019 Feb;566(7743):218-223. doi: 10.1038/s41586-019-0908-x. Epub 2019 Feb, 4. PMID:30718774<ref>PMID:30718774</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ny1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Doudna, J A]]
[[Category: Deltaproteobacteria bacterium]]
[[Category: Liu, J J]]
[[Category: Large Structures]]
[[Category: Nogales, E]]
[[Category: Doudna JA]]
[[Category: Orlova, N]]
[[Category: Liu JJ]]
[[Category: Casx]]
[[Category: Nogales E]]
[[Category: Crispr]]
[[Category: Orlova N]]
[[Category: Rna binding protein-rna-dna complex]]
[[Category: Sgrna]]
[[Category: Target dna]]
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