6mzb

Cryo-EM structure of phosphodiesterase 6

Loading... Status: waiting for Mol* initialization Structural highlights 6mzb is a 4 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.4Å Ligands: 35G, MG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PDE6B_BOVIN This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme. Publication Abstract from PubMed Cyclic nucleotide phosphodiesterases (PDEs) work in conjunction with adenylate/guanylate cyclases to regulate the key second messengers of G protein-coupled receptor signaling. Previous attempts to determine the full-length structure of PDE family members at high-resolution have been hindered by structural flexibility, especially in their linker regions and N- and C-terminal ends. Therefore, most structure-activity relationship studies have so far focused on truncated and conserved catalytic domains rather than the regulatory domains that allosterically govern the activity of most PDEs. Here, we used single-particle cryo-electron microscopy to determine the structure of the full-length PDE6alphabeta2gamma complex. The final density map resolved at 3.4 A reveals several previously unseen structural features, including a coiled N-terminal domain and the interface of PDE6gamma subunits with the PDE6alphabeta heterodimer. Comparison of the PDE6alphabeta2gamma complex with the closed state of PDE2A sheds light on the conformational changes associated with the allosteric activation of type I PDEs. , PMID:30820458 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Phosphodiesterase 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA