1ft9: Difference between revisions

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==STRUCTURE OF THE REDUCED (FEII) CO-SENSING PROTEIN FROM R. RUBRUM==
<!-- STRUCTURE TEMPLATE BEGIN -->
<StructureSection load='1ft9' size='340' side='right'caption='[[1ft9]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
{{Structure
== Structural highlights ==
|PDB= 1ft9 |SIZE=350|CAPTION= <scene name='initialview01'>1ft9</scene>, resolution 2.6&Aring;
<table><tr><td colspan='2'>[[1ft9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodospirillum_rubrum Rhodospirillum rubrum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FT9 FirstGlance]. <br>
|SITE=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
|ACTIVITY=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ft9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ft9 OCA], [https://pdbe.org/1ft9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ft9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ft9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ft9 ProSAT]</span></td></tr>
|GENE=  
</table>
|DOMAIN=
== Function ==
|RELATEDENTRY=
[https://www.uniprot.org/uniprot/P72322_RHORU P72322_RHORU]
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ft9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ft9 OCA], [http://www.ebi.ac.uk/pdbsum/1ft9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ft9 RCSB]</span>
== Evolutionary Conservation ==
}}
[[Image:Consurf_key_small.gif|200px|right]]
<!-- STRUCTURE TEMPLATE END -->
Check<jmol>
 
  <jmolCheckbox>
'''STRUCTURE OF THE REDUCED (FEII) CO-SENSING PROTEIN FROM R. RUBRUM'''
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/1ft9_consurf.spt"</scriptWhenChecked>
 
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Overview==
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ft9 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CooA is a homodimeric transcription factor that belongs to the catabolite activator protein (CAP) family. Binding of CO to the heme groups of CooA leads to the transcription of genes involved in CO oxidation in Rhodospirillum rubrum. The 2.6 A structure of reduced (Fe2+) CooA reveals that His 77 in both subunits provides one heme ligand while the N-terminal nitrogen of Pro 2 from the opposite subunit provides the other ligand. A structural comparison of CooA in the absence of effector and DNA (off state) with that of CAP in the effector and DNA bound state (on state) leads to a plausible model for the mechanism of allosteric control in this class of proteins as well as the CO dependent activation of CooA.
CooA is a homodimeric transcription factor that belongs to the catabolite activator protein (CAP) family. Binding of CO to the heme groups of CooA leads to the transcription of genes involved in CO oxidation in Rhodospirillum rubrum. The 2.6 A structure of reduced (Fe2+) CooA reveals that His 77 in both subunits provides one heme ligand while the N-terminal nitrogen of Pro 2 from the opposite subunit provides the other ligand. A structural comparison of CooA in the absence of effector and DNA (off state) with that of CAP in the effector and DNA bound state (on state) leads to a plausible model for the mechanism of allosteric control in this class of proteins as well as the CO dependent activation of CooA.


==About this Structure==
Structure of the CO sensing transcription activator CooA.,Lanzilotta WN, Schuller DJ, Thorsteinsson MV, Kerby RL, Roberts GP, Poulos TL Nat Struct Biol. 2000 Oct;7(10):876-80. PMID:11017196<ref>PMID:11017196</ref>
1FT9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodospirillum_rubrum Rhodospirillum rubrum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FT9 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of the CO sensing transcription activator CooA., Lanzilotta WN, Schuller DJ, Thorsteinsson MV, Kerby RL, Roberts GP, Poulos TL, Nat Struct Biol. 2000 Oct;7(10):876-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11017196 11017196]
</div>
<div class="pdbe-citations 1ft9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rhodospirillum rubrum]]
[[Category: Rhodospirillum rubrum]]
[[Category: Single protein]]
[[Category: Kirby B]]
[[Category: Kirby, B.]]
[[Category: Lanzilotta WN]]
[[Category: Lanzilotta, W N.]]
[[Category: Poulos TL]]
[[Category: Poulos, T L.]]
[[Category: Roberts GP]]
[[Category: Roberts, G P.]]
[[Category: Schuller DJ]]
[[Category: Schuller, D J.]]
[[Category: Thorsteinsson MV]]
[[Category: Thorsteinsson, M V.]]
[[Category: Carbon monoxide]]
[[Category: Catabolite gene activator protein]]
[[Category: Heme sensor]]
 
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