6o8g: Difference between revisions
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New page: '''Unreleased structure''' The entry 6o8g is ON HOLD Authors: Lee, S.-J., Sung, R.-J., Verdine, G.L. Description: Crystal structure of UvrB bound to fully duplex DNA [[Category: Unrele... |
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The | ==Crystal structure of UvrB bound to fully duplex DNA== | ||
<StructureSection load='6o8g' size='340' side='right'caption='[[6o8g]], [[Resolution|resolution]] 2.64Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6o8g]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_caldotenax Bacillus caldotenax] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O8G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O8G FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.64Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o8g OCA], [https://pdbe.org/6o8g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o8g RCSB], [https://www.ebi.ac.uk/pdbsum/6o8g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o8g ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/UVRB_BACCA UVRB_BACCA] The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage (By similarity).[HAMAP-Rule:MF_00204] | |||
==See Also== | |||
*[[UvrABC|UvrABC]] | |||
__TOC__ | |||
[[Category: | </StructureSection> | ||
[[Category: Lee | [[Category: Large Structures]] | ||
[[Category: | [[Category: Synthetic construct]] | ||
[[Category: | [[Category: Lee S-J]] | ||
[[Category: Sung R-J]] | |||
[[Category: Verdine GL]] | |||
Latest revision as of 14:51, 13 March 2024
Crystal structure of UvrB bound to fully duplex DNA
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