2pr8: Difference between revisions

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==crystal structure of aminoglycoside N-acetyltransferase AAC(6')-Ib11==
The line below this paragraph, containing "STRUCTURE_2pr8", creates the "Structure Box" on the page.
<StructureSection load='2pr8' size='340' side='right'caption='[[2pr8]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2pr8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PR8 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr>
{{STRUCTURE_2pr8|  PDB=2pr8  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pr8 OCA], [https://pdbe.org/2pr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pr8 RCSB], [https://www.ebi.ac.uk/pdbsum/2pr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pr8 ProSAT]</span></td></tr>
 
</table>
'''crystal structure of aminoglycoside N-acetyltransferase AAC(6')-Ib11'''
== Function ==
 
[https://www.uniprot.org/uniprot/Q8GLI5_SALTM Q8GLI5_SALTM]
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
The emergence of multi-resistant pathogenic bacteria is a worldwide health issue. Recently, clinical variants of a single antibiotic-modifying acetyltransferase, AAC(6')-Ib-a variant of aminoglycoside 6'-N-acetyltransferase-have been identified that confer extended resistance to most aminoglycosides and, more surprisingly, to structurally unrelated fluoroquinolones. The corresponding gene is carried by mobile genetic elements and is present in most multi-resistant pathogenic strains, hence making it a serious threat to current therapies. Here, we report the crystal structures of both narrow- and broad-spectrum resistance variants of this enzyme, which reveal the structural basis for the emergence of extended resistance. The active site shows an important plasticity and has adapted to new substrates by a large-scale gaping process. We have also obtained co-crystals with both substrates, and with a simple transition state analogue, which provides new clues for the design of inhibitors of this resistance mechanism.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/2pr8_consurf.spt"</scriptWhenChecked>
2PR8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PR8 OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
Enzyme structural plasticity and the emergence of broad-spectrum antibiotic resistance., Maurice F, Broutin I, Podglajen I, Benas P, Collatz E, Dardel F, EMBO Rep. 2008 Apr;9(4):344-349. Epub 2008 Feb 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18292754 18292754]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pr8 ConSurf].
[[Category: Salmonella typhimurium]]
<div style="clear:both"></div>
[[Category: Single protein]]
__TOC__
[[Category: Benas, P.]]
</StructureSection>
[[Category: Broutin, I.]]
[[Category: Large Structures]]
[[Category: Collatz, E.]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
[[Category: Dardel, F.]]
[[Category: Benas P]]
[[Category: Maurice, F.]]
[[Category: Broutin I]]
[[Category: Podglajen, I.]]
[[Category: Collatz E]]
[[Category: Aminoglycoside acetyltransferase]]
[[Category: Dardel F]]
[[Category: Gnat]]
[[Category: Maurice F]]
[[Category: Podglajen I]]
Retrieved from "https://proteopedia.org/2pr8"