6jp2: Difference between revisions
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==Crystal structure of pyrrolysyl-tRNA synthetase from Methanomethylophilus alvus== | |||
<StructureSection load='6jp2' size='340' side='right'caption='[[6jp2]], [[Resolution|resolution]] 2.27Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6jp2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Methanomethylophilus_alvus Candidatus Methanomethylophilus alvus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JP2 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.272Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jp2 OCA], [https://pdbe.org/6jp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jp2 RCSB], [https://www.ebi.ac.uk/pdbsum/6jp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jp2 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/M9SC49_METAX M9SC49_METAX] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Pyrrolysyl-tRNA synthetase (PylRS)/tRNA(Pyl) pairs from Methanosarcina mazei and Methanosarcina barkeri are widely used for site-specific incorporations of non-canonical amino acids into proteins (genetic code expansion). In this study, we achieved the full productivity of cell-free protein synthesis for difficult, bulky non-canonical amino acids, such as N(epsilon)-((((E)-cyclooct-2-en-1-yl)oxy)carbonyl)-l-lysine (TCO*Lys), by using Methanomethylophilus alvus PylRS. First, based on the crystal structure of M. alvus PylRS, the productivities for various non-canonical amino acids were greatly increased by rational engineering of the amino acid-binding pocket. The productivities were further enhanced by using a much higher concentration of PylRS over that of M. mazei PylRS, or by mutating the outer layer of the amino acid-binding pocket. Thus, we achieved full productivity even for TCO*Lys. The quantity and quality of the cell-free-produced antibody fragment containing TCO*Lys were drastically improved. These results demonstrate the importance of full productivity for the expanded genetic code. | |||
Fully Productive Cell-Free Genetic Code Expansion by Structure-Based Engineering of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase.,Seki E, Yanagisawa T, Kuratani M, Sakamoto K, Yokoyama S ACS Synth Biol. 2020 Apr 17;9(4):718-732. doi: 10.1021/acssynbio.9b00288. Epub, 2020 Mar 17. PMID:32182048<ref>PMID:32182048</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6jp2" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Candidatus Methanomethylophilus alvus]] | |||
[[Category: Large Structures]] | |||
[[Category: Kuratani M]] | |||
[[Category: Yanagisawa T]] | |||
[[Category: Yokoyama S]] | |||
Latest revision as of 10:17, 22 November 2023
Crystal structure of pyrrolysyl-tRNA synthetase from Methanomethylophilus alvus
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