6od9: Difference between revisions
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New page: '''Unreleased structure''' The entry 6od9 is ON HOLD Authors: Jones, C.P., Tran, B., Ferre-D'Amare, A.R. Description: F. ulcerans ZTP riboswitch XFEL [[Category: Unreleased Structures]... |
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==Co-crystal structure of the Fusobacterium ulcerans ZTP riboswitch using an X-ray free-electron laser== | |||
<StructureSection load='6od9' size='340' side='right'caption='[[6od9]], [[Resolution|resolution]] 4.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6od9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusobacterium_ulcerans Fusobacterium ulcerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OD9 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.102Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMZ:AMINOIMIDAZOLE+4-CARBOXAMIDE+RIBONUCLEOTIDE'>AMZ</scene>, <scene name='pdbligand=CS:CESIUM+ION'>CS</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6od9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6od9 OCA], [https://pdbe.org/6od9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6od9 RCSB], [https://www.ebi.ac.uk/pdbsum/6od9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6od9 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Riboswitches are conformationally dynamic RNAs that regulate gene expression by binding specific small molecules. ZTP riboswitches bind the purine-biosynthetic intermediate 5-aminoimidazole-4-carboxamide riboside 5'-monophosphate (ZMP) and its triphosphorylated form (ZTP). Ligand binding to this riboswitch ultimately upregulates genes involved in folate and purine metabolism. Using an X-ray free-electron laser (XFEL), the room-temperature structure of the Fusobacterium ulcerans ZTP riboswitch bound to ZMP has now been determined at 4.1 A resolution. This model, which was refined against a data set from approximately 750 diffraction images (each from a single crystal), was found to be consistent with that previously obtained from data collected at 100 K using conventional synchrotron X-radiation. These experiments demonstrate the feasibility of time-resolved XFEL experiments to understand how the ZTP riboswitch accommodates cognate ligand binding. | |||
Co-crystal structure of the Fusobacterium ulcerans ZTP riboswitch using an X-ray free-electron laser.,Jones C, Tran B, Conrad C, Stagno J, Trachman R 3rd, Fischer P, Meents A, Ferre-D'Amare A Acta Crystallogr F Struct Biol Commun. 2019 Jul 1;75(Pt 7):496-500. doi:, 10.1107/S2053230X19008549. Epub 2019 Jun 28. PMID:31282869<ref>PMID:31282869</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[ | </div> | ||
[[Category: | <div class="pdbe-citations 6od9" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: Ferre-D'Amare | ==See Also== | ||
*[[Riboswitch 3D structures|Riboswitch 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Fusobacterium ulcerans]] | |||
[[Category: Large Structures]] | |||
[[Category: Ferre-D'Amare AR]] | |||
[[Category: Jones CP]] | |||
[[Category: Tran B]] | |||
Latest revision as of 07:08, 11 October 2023
Co-crystal structure of the Fusobacterium ulcerans ZTP riboswitch using an X-ray free-electron laser
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