5vjw: Difference between revisions

Latest revision as of 12:38, 13 August 2026

Arabidopsis thaliana Rhizobiales-like phosphatase 2 complexed with tungstate

Structural highlights

5vjw is a 1 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Ligands:	WO4, ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RLPH2_ARATH Protein phosphatase that dephosphorylates specifically tyrosine-phosphorylated peptides; especially active on dual-phosphorylated substrates containing a phosphothreonine-X-phosphotyrosine motif.^[1]

Publication Abstract from PubMed

Despite belonging to the phosphoserine- and phosphothreonine-specific phosphoprotein phosphatase (PPP) family, Arabidopsis thaliana Rhizobiales-like phosphatase 2 (RLPH2) strongly prefers substrates bearing phosphorylated tyrosine residues. We solved the structures of RLPH2 crystallized in the presence or absence of sodium tungstate. These structures revealed the presence of a central domain that forms a binding site for two divalent metal ions that closely resembles that of other PPP-family enzymes. Unique structural elements from two flanking domains suggest a mechanism for the selective dephosphorylation of phosphotyrosine residues. Cocrystallization with the phosphate mimetic tungstate also suggests how positively charged residues that are highly conserved in the RLPH2 class form an additional pocket that is specific for a phosphothreonine residue located near the phosphotyrosine residue that is bound to the active site. Site-directed mutagenesis confirmed that this auxiliary recognition element facilitates the recruitment of dual-phosphorylated substrates containing a pTxpY motif.

Structural basis for the preference of the Arabidopsis thaliana phosphatase RLPH2 for tyrosine-phosphorylated substrates.,Labandera AM, Uhrig RG, Colville K, Moorhead GB, Ng KKS Sci Signal. 2018 Apr 3;11(524). pii: 11/524/eaan8804. doi:, 10.1126/scisignal.aan8804. PMID:29615518^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Uhrig RG, Labandera AM, Muhammad J, Samuel M, Moorhead GB. Rhizobiales-like Phosphatase 2 from Arabidopsis thaliana Is a Novel Phospho-tyrosine-specific Phospho-protein Phosphatase (PPP) Family Protein Phosphatase. J Biol Chem. 2016 Mar 11;291(11):5926-5934. PMID:26742850 doi:10.1074/jbc.M115.683656
↑ Labandera AM, Uhrig RG, Colville K, Moorhead GB, Ng KKS. Structural basis for the preference of the Arabidopsis thaliana phosphatase RLPH2 for tyrosine-phosphorylated substrates. Sci Signal. 2018 Apr 3;11(524). pii: 11/524/eaan8804. doi:, 10.1126/scisignal.aan8804. PMID:29615518 doi:https://dx.doi.org/10.1126/scisignal.aan8804

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Uhrig RG, Labandera AM, Muhammad J, Samuel M, Moorhead GB. Rhizobiales-like Phosphatase 2 from Arabidopsis thaliana Is a Novel Phospho-tyrosine-specific Phospho-protein Phosphatase (PPP) Family Protein Phosphatase. J Biol Chem. 2016 Mar 11;291(11):5926-5934. PMID:26742850 doi:10.1074/jbc.M115.683656

[2] Labandera AM, Uhrig RG, Colville K, Moorhead GB, Ng KKS. Structural basis for the preference of the Arabidopsis thaliana phosphatase RLPH2 for tyrosine-phosphorylated substrates. Sci Signal. 2018 Apr 3;11(524). pii: 11/524/eaan8804. doi:, 10.1126/scisignal.aan8804. PMID:29615518 doi:https://dx.doi.org/10.1126/scisignal.aan8804

[1]

[2]

@@ Line 3: / Line 3: @@
 <StructureSection load='5vjw' size='340' side='right'caption='[[5vjw]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5vjw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VJW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5vjw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VJW FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5vjv|5vjv]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">T22K18.22, At3g09970 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vjw OCA], [https://pdbe.org/5vjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vjw RCSB], [https://www.ebi.ac.uk/pdbsum/5vjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vjw ProSAT]</span></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vjw OCA], [http://pdbe.org/5vjw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vjw RCSB], [http://www.ebi.ac.uk/pdbsum/5vjw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vjw ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/RLPH2_ARATH RLPH2_ARATH] Protein phosphatase that dephosphorylates specifically tyrosine-phosphorylated peptides; especially active on dual-phosphorylated substrates containing a phosphothreonine-X-phosphotyrosine motif.<ref>PMID:26742850</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Arath]]
+[[Category: Arabidopsis thaliana]]
 [[Category: Large Structures]]
-[[Category: Protein-tyrosine-phosphatase]]
+[[Category: Labandera A]]
-[[Category: Labandera, A]]
+[[Category: Moorhead G]]
-[[Category: Moorhead, G]]
+[[Category: Ng KKS]]
-[[Category: Ng, K K.S]]
-[[Category: Arabidopsis thaliana]]
-[[Category: Hydrolase]]
-[[Category: Phosphoprotein phosphatase]]
-[[Category: Protein phosphatase]]
-[[Category: Protein phosphorylation]]
-[[Category: Protein tyrosine phosphatase]]
-[[Category: Tungstate]]

5vjw: Difference between revisions

Latest revision as of 12:38, 13 August 2026

Arabidopsis thaliana Rhizobiales-like phosphatase 2 complexed with tungstate

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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