6jrf: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "6jrf" [edit=sysop:move=sysop] |
No edit summary |
||
| (3 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==Crystal structure of ZmMoc1-Holliday junction Complex in the presence of Calcium== | ||
<StructureSection load='6jrf' size='340' side='right'caption='[[6jrf]], [[Resolution|resolution]] 2.05Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6jrf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JRF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JRF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.047Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jrf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jrf OCA], [https://pdbe.org/6jrf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jrf RCSB], [https://www.ebi.ac.uk/pdbsum/6jrf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jrf ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B4FCI7_MAIZE B4FCI7_MAIZE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The Holliday junction (HJ) is a key intermediate during homologous recombination and DNA double-strand break repair. Timely HJ resolution by resolvases is critical for maintaining genome stability. The mechanisms underlying sequence-specific substrate recognition and cleavage by resolvases remain elusive. The monokaryotic chloroplast 1 protein (MOC1) specifically cleaves four-way DNA junctions in a sequence-specific manner. Here, we report the crystal structures of MOC1 from Zea mays, alone or bound to HJ DNA. MOC1 uses a unique beta-hairpin to embrace the DNA junction. A base-recognition motif specifically interacts with the junction center, inducing base flipping and pseudobase-pair formation at the strand-exchanging points. Structures of MOC1 bound to HJ and different metal ions support a two-metal ion catalysis mechanism. Further molecular dynamics simulations and biochemical analyses reveal a communication between specific substrate recognition and metal ion-dependent catalysis. Our study thus provides a mechanism for how a resolvase turns substrate specificity into catalytic efficiency. | |||
Structural basis of sequence-specific Holliday junction cleavage by MOC1.,Lin H, Zhang D, Zuo K, Yuan C, Li J, Huang M, Lin Z Nat Chem Biol. 2019 Oct 14. pii: 10.1038/s41589-019-0377-4. doi:, 10.1038/s41589-019-0377-4. PMID:31611704<ref>PMID:31611704</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Lin | <div class="pdbe-citations 6jrf" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: Yuan | ==See Also== | ||
[[Category: | *[[Resolvase 3D structures|Resolvase 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Zea mays]] | |||
[[Category: Lin H]] | |||
[[Category: Lin Z]] | |||
[[Category: Yuan C]] | |||
[[Category: Zhang D]] | |||
Latest revision as of 10:18, 22 November 2023
Crystal structure of ZmMoc1-Holliday junction Complex in the presence of Calcium
| ||||||||||||
