6r7m: Difference between revisions
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==Tobacco Mosaic Virus (TMV)== | |||
<SX load='6r7m' size='340' side='right' viewer='molstar' caption='[[6r7m]], [[Resolution|resolution]] 1.92Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6r7m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Tobacco_mosaic_virus Tobacco mosaic virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R7M OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6R7M FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6r7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r7m OCA], [http://pdbe.org/6r7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r7m RCSB], [http://www.ebi.ac.uk/pdbsum/6r7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r7m ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
High-resolution structural information is essential to understand protein function. Protein-structure determination needs a considerable amount of protein, which can be challenging to produce, often involving harsh and lengthy procedures. In contrast, the several thousand to a few million protein particles required for structure determination by cryogenic electron microscopy (cryo-EM) can be provided by miniaturized systems. Here, we present a microfluidic method for the rapid isolation of a target protein and its direct preparation for cryo-EM. Less than 1 muL of cell lysate is required as starting material to solve the atomic structure of the untagged, endogenous human 20S proteasome. Our work paves the way for high-throughput structure determination of proteins from minimal amounts of cell lysate and opens more opportunities for the isolation of sensitive, endogenous protein complexes. | |||
Microfluidic protein isolation and sample preparation for high-resolution cryo-EM.,Schmidli C, Albiez S, Rima L, Righetto R, Mohammed I, Oliva P, Kovacik L, Stahlberg H, Braun T Proc Natl Acad Sci U S A. 2019 Jul 10. pii: 1907214116. doi:, 10.1073/pnas.1907214116. PMID:31292253<ref>PMID:31292253</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6r7m" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Large Structures]] | |||
[[Category: Tobacco mosaic virus]] | |||
[[Category: Albiez, S]] | |||
[[Category: Braun, T]] | |||
[[Category: Kovacik, L]] | |||
[[Category: Mohammed, I]] | |||
[[Category: Oliva, P]] | |||
[[Category: Righetto, R]] | [[Category: Righetto, R]] | ||
[[Category: | [[Category: Rima, L]] | ||
[[Category: Schmidli, C]] | [[Category: Schmidli, C]] | ||
[[Category: Stahlberg, H]] | [[Category: Stahlberg, H]] | ||
[[Category: | [[Category: Cryo-em]] | ||
[[Category: Cryowriter]] | |||
[[Category: Microfluidic]] | |||
[[Category: Mosaic]] | |||
[[Category: Tmv]] | |||
[[Category: Tobacco]] | |||
[[Category: Virus]] | |||
Latest revision as of 05:20, 11 April 2020
Tobacco Mosaic Virus (TMV)
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