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| <StructureSection load='6j5g' size='340' side='right'caption='[[6j5g]], [[Resolution|resolution]] 3.29Å' scene=''> | | <StructureSection load='6j5g' size='340' side='right'caption='[[6j5g]], [[Resolution|resolution]] 3.29Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6j5g]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Neuv Neuv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J5G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J5G FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6j5g]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Tick-borne_encephalitis_virus_(WESTERN_SUBTYPE) Tick-borne encephalitis virus (WESTERN SUBTYPE)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J5G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J5G FirstGlance]. <br> |
| </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j5g OCA], [http://pdbe.org/6j5g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j5g RCSB], [http://www.ebi.ac.uk/pdbsum/6j5g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j5g ProSAT]</span></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.291Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j5g OCA], [https://pdbe.org/6j5g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j5g RCSB], [https://www.ebi.ac.uk/pdbsum/6j5g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j5g ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function ==
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| [[http://www.uniprot.org/uniprot/POLG_TBEVW POLG_TBEVW]] Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (By similarity). prM acts as a chaperone for envelope protein E during intracellular virion assembly by masking and inactivating envelope protein E fusion peptide. prM is matured in the last step of virion assembly, presumably to avoid catastrophic activation of the viral fusion peptide induced by the acidic pH of the trans-Golgi network. After cleavage by host furin, the pr peptide is released in the extracellular medium and small envelope protein M and envelope protein E homodimers are dissociated (By similarity). Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity). Non-structural protein 1 is involved in virus replication and regulation of the innate immune response (By similarity). Non-structural protein 2A may be involved viral RNA replication and capsid assembly (Potential). Non-structural protein 2B is a required cofactor for the serine protease function of NS3 (By similarity). Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, performs its autocleavage and cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B-NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction (By similarity). Non-structural protein 4A induces host endoplasmic reticulum membrane rearrangements leading to the formation of virus-induced membranous vesicles hosting the dsRNA and polymerase, functioning as a replication complex. NS4A might also regulate the ATPase activity of the NS3 helicase (By similarity). Peptide 2k functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter (By similarity). RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2'-O positions. Besides its role in genome replication, also prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) signaling pathway. Inhibits host SCRIB and prevents activation of downstream JAK-STAT signaling pathway (By similarity).
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| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| ==See Also== | | ==See Also== |
| *[[Antibody 3D structures|Antibody 3D structures]] | | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] |
| | *[[3D structures of non-human antibody|3D structures of non-human antibody]] |
| == References == | | == References == |
| <references/> | | <references/> |
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| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Lk3 transgenic mice]] | | [[Category: Mus musculus]] |
| [[Category: Neuv]]
| | [[Category: Dai L]] |
| [[Category: Dai, L]] | | [[Category: Gao GF]] |
| [[Category: Gao, G F]] | | [[Category: Gould EA]] |
| [[Category: Gould, E A]] | | [[Category: Peng R]] |
| [[Category: Peng, R]] | | [[Category: Qi J]] |
| [[Category: Qi, J]] | | [[Category: Tien P]] |
| [[Category: Tien, P]] | | [[Category: Yang X]] |
| [[Category: Yang, X]] | |
| [[Category: Antiviral protein]]
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| [[Category: Flavivirus envelope protein]]
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| [[Category: Protective and neutralizing antibody]]
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| [[Category: Viral protein-immune system complex]]
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