6om5: Difference between revisions
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New page: '''Unreleased structure''' The entry 6om5 is ON HOLD Authors: Torrado, M., Walshe, J.L., Mackay, J.P., Guss, J.M., Gell, D.A. Description: Structure of a haemophore from Haemophilus ha... |
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==Structure of a haemophore from Haemophilus haemolyticus== | |||
<StructureSection load='6om5' size='340' side='right'caption='[[6om5]], [[Resolution|resolution]] 1.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6om5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OM5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OM5 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6om5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6om5 OCA], [https://pdbe.org/6om5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6om5 RCSB], [https://www.ebi.ac.uk/pdbsum/6om5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6om5 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0M3G7Q5_HAEHA A0A0M3G7Q5_HAEHA] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Commensal bacteria serve as an important line of defense against colonisation by opportunisitic pathogens, but the underlying molecular mechanisms remain poorly explored. Here, we show that strains of a commensal bacterium, Haemophilus haemolyticus, make hemophilin, a heme-binding protein that inhibits growth of the opportunistic pathogen, non-typeable Haemophilus influenzae (NTHi) in culture. We purified the NTHi-inhibitory protein from H. haemolyticus and identified the hemophilin gene using proteomics and a gene knockout. An x-ray crystal structure of recombinant hemophilin shows that the protein does not belong to any of the known heme-binding protein folds, suggesting that it evolved independently. Biochemical characterisation shows that heme can be captured in the ferrous or ferric state, and with a variety of small heme-ligands bound, suggesting that hemophilin could function under a range of physiological conditions. Hemophilin knockout bacteria show a limited capacity to utilise free heme for growth. Our data suggest that hemophilin is a hemophore and that inhibition of NTHi occurs by heme starvation, raising the possibility that competition from hemophilin-producing H. haemolyticus could antagonise NTHi colonisation in the respiratory tract. | |||
A heme-binding protein produced by Haemophilus haemolyticus inhibits non-typeable Haemophilus influenzae.,Latham RD, Torrado M, Atto B, Walshe JL, Wilson R, Guss JM, Mackay JP, Tristram S, Gell DA Mol Microbiol. 2019 Nov 19. doi: 10.1111/mmi.14426. PMID:31742788<ref>PMID:31742788</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6om5" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Haemophilus haemolyticus]] | |||
[[Category: Large Structures]] | |||
[[Category: Gell DA]] | |||
[[Category: Guss JM]] | |||
[[Category: Mackay JP]] | |||
[[Category: Torrado M]] | |||
[[Category: Walshe JL]] | |||
Latest revision as of 13:39, 13 August 2026
Structure of a haemophore from Haemophilus haemolyticus
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