4q2l

Atomic Resolution Structure of the E. coli YajR Transporter YAM Domain

spinqualitylabelsall models 4q2l, resolution 1.07Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4q2l is a 1 chain structure with sequence from Escherichia coli BL21(DE3). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.071Å Ligands: ACY, CD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed YajR is an Escherichia coli transporter that belongs to the major facilitator superfamily. Unlike most MFS transporters, YajR contains a carboxyl terminal, cytosolic domain of 67 amino acid residues termed YAM domain. Although it is speculated that the function of this small soluble domain is to regulate the conformational change of the 12-helix transmembrane domain, its precise regulatory role remains unclear. Here, we report the crystal structure of the YAM domain at 1.07-A resolution, along with its structure determined using nuclear magnetic resonance. Detailed analysis of the high resolution structure revealed a symmetrical dimer in which a belt of well-ordered poly-pentagonal water molecules is embedded. A mutagenesis experiment and a thermal stability assay were used to analyze the putative role of this dimerization in response to changes in halogen concentration. Atomic resolution structure of the E. coli YajR transporter YAM domain.,Jiang D, Zhao Y, Fan J, Liu X, Wu Y, Feng W, Zhang XC Biochem Biophys Res Commun. 2014 Jun 19. pii: S0006-291X(14)01127-9. doi:, 10.1016/j.bbrc.2014.06.053. PMID:24952155 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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