6rj7: Difference between revisions
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New page: '''Unreleased structure''' The entry 6rj7 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures |
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==Crystal structure of the 19F labelled OXA-48== | |||
<StructureSection load='6rj7' size='340' side='right'caption='[[6rj7]], [[Resolution|resolution]] 1.73Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6rj7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RJ7 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7300223Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=K5H:(2~{R})-2-azanyl-3-[3,3,3-tris(fluoranyl)-2,2-bis(oxidanyl)propyl]sulfanyl-propanoic+acid'>K5H</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rj7 OCA], [https://pdbe.org/6rj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rj7 RCSB], [https://www.ebi.ac.uk/pdbsum/6rj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rj7 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q6XEC0_KLEPN Q6XEC0_KLEPN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacterial production of beta-lactamases with carbapenemase activity is a global health threat. The active sites of class D carbapenemases such as OXA-48, which is of major clinical importance, uniquely contain a carbamylated lysine residue which is essential for catalysis. Although there is significant interest in characterizing this post-translational modification, and it is a promising inhibition target, protein carbamylation is challenging to monitor in solution. We report the use of 19F-NMR spectroscopy to monitor the carbamylation state of 19F-labelled OXA-48. This method was used to investigate the interactions of OXA-48 with clinically used serine beta- lactamase inhibitors, including avibactam and vaborbactam. Crystallographic studies on 19F-labelled OXA-48 provide a structural rationale for the sensitivity of the 19F-label to active site interactions. The overall results demonstrate the use of 19F-NMR to monitor reversible covalent post-translational modifications. | |||
19F-NMR Monitoring of Reversible Protein Post-Translational Modifications: Class D beta-Lactamase Carbamylation and Inhibition.,van Groesen E, Lohans CT, Brem J, Aertker KM, Claridge TD, Schofield C Chemistry. 2019 Jul 16. doi: 10.1002/chem.201902529. PMID:31310409<ref>PMID:31310409</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6rj7" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Klebsiella pneumoniae]] | |||
[[Category: Large Structures]] | |||
[[Category: Brem J]] | |||
[[Category: Lohans C]] | |||
[[Category: Schofield C]] | |||
Latest revision as of 12:21, 24 January 2024
Crystal structure of the 19F labelled OXA-48
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