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| <StructureSection load='4rhf' size='340' side='right'caption='[[4rhf]], [[Resolution|resolution]] 1.76Å' scene=''> | | <StructureSection load='4rhf' size='340' side='right'caption='[[4rhf]], [[Resolution|resolution]] 1.76Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4rhf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Colp3 Colp3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RHF FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4rhf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Colwellia_psychrerythraea_34H Colwellia psychrerythraea 34H]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RHF FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.764Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4rhe|4rhe]]</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ubiX, CPS_0408 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=167879 COLP3])</td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhf OCA], [https://pdbe.org/4rhf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rhf RCSB], [https://www.ebi.ac.uk/pdbsum/4rhf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rhf ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhf OCA], [http://pdbe.org/4rhf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rhf RCSB], [http://www.ebi.ac.uk/pdbsum/4rhf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rhf ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/Q489U8_COLP3 Q489U8_COLP3] |
| The ubiX gene of Colwellia psychrerythraea strain 34H encodes a 3-octaprenyl-4-hydroxybenzoate carboxylase (CpsUbiX, UniProtKB code: Q489U8) that is involved in the third step of the ubiquinone biosynthesis pathway and harbors a flavin mononucleotide (FMN) as a potential cofactor. Here, we report the crystal structures of two forms of CpsUbiX: an FMN-bound wild type form and an FMN-unbound V47S mutant form. CpsUbiX is a dodecameric enzyme, and each monomer possesses a typical Rossmann-fold structure. The FMN-binding domain of UbiX is composed of three neighboring subunits. The highly conserved Gly15, Ser41, Val47, and Tyr171 residues play important roles in FMN binding. Structural comparison of the FMN-bound wild type form with the FMN-free form reveals a significant conformational difference in the C-terminal loop region (comprising residues 170-176 and 195-206). Subsequent computational modeling and liposome binding assay both suggest that the conformational flexibility observed in the C-terminal loops plays an important role in substrate and lipid bindings. The crystal structures presented in this work provide structural framework and insights into the catalytic mechanism of CpsUbiX.
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| Crystal structure of UbiX, an aromatic acid decarboxylase from the psychrophilic bacterium Colwellia psychrerythraea that undergoes FMN-induced conformational changes.,Do H, Kim SJ, Lee CW, Kim HW, Park HH, Kim HM, Park H, Park H, Lee JH Sci Rep. 2015 Feb 3;5:8196. doi: 10.1038/srep08196. PMID:25645665<ref>PMID:25645665</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 4rhf" style="background-color:#fffaf0;"></div>
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| == References ==
| |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Colp3]] | | [[Category: Colwellia psychrerythraea 34H]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Do, H]] | | [[Category: Do H]] |
| [[Category: Kim, H M]] | | [[Category: Kim H-W]] |
| [[Category: Kim, H W]] | | [[Category: Kim HM]] |
| [[Category: Kim, S J]] | | [[Category: Kim SJ]] |
| [[Category: Lee, C W]] | | [[Category: Lee CW]] |
| [[Category: Lee, J H]] | | [[Category: Lee JH]] |
| [[Category: Park, H]] | | [[Category: Park H]] |
| [[Category: Park, H H]] | | [[Category: Park HH]] |
| [[Category: Park, H J]] | | [[Category: Park HJ]] |
| [[Category: Decarboxylation]]
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| [[Category: Lyase]]
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| [[Category: Rossmann fold]]
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