Sandbox Reserved 1546: Difference between revisions

(8 intermediate revisions by the same user not shown)

Line 20:

== Structural Highlights & Disease ==

===Structure===

The enzyme, 5h86, can be expressed in ''E. coli'' and is found in humans <ref name = "Structural basis for acyl-group discrimination by human Gcn5L2"/>. The characteristics of this 5h86 Human Gcn5 enzyme bound to butyrl-coA can be summarized in the table below. Clicking on each green linked element will convey important structural information of the enzyme.

Line 41:

<tr id='activity'><td class="sblockLbl">Type of Enzymatic Activity:</td><td class="sblockDat">[http://en.wikipedia.org/wiki/Histone_acetyltransferase Histone acetyltransferase]</td></tr>

<tr id='resources'><td class="sblockLbl">Resources:</td><td class="sblockDat">[http://www.rcsb.org/pdb/explore.do?structureId=5h86 RCSB]</td></tr>

<tr id='resources'><td class="sblockLbl">Resources:</td><td class="sblockDat">[http://www.rcsb.org/pdb/explore.do?structureId=5h86 RCSB 5h86 PDB], [http://proteopedia.org/wiki/index.php/5h86#cite_note-0 5h86 Proteopedia Page]</td></tr>

</table></center>

Line 47:

Under a "backbone" representation, the <scene name='80/806432/Unique_protein_chain/1'>one unique protein chain</scene> of the 5h86 enzyme can be visualized. The structure of this molecule is also composed of <scene name='80/806432/Secondary_structures/1'>alpha helices (magenta) and beta strands (gold)</scene>.

The ~~168~~ <scene name='80/806432/Aa_residues/1'>amino acid residues</scene> can be visualized by the ~~gold circles~~ of the ~~structure~~.

The <scene name='80/806432/Aa_residues/3'>168 amino acid residues</scene> can be visualized by the green ball and stick structures of the molecule.

There are <scene name='80/806432/Ligands/1'>two ligand binding sites</scene>, which can be represented in green and the protein structure is represented by a khaki color. The active site of Gcn5 contains two binding grooves where acetyl-CoA and peptide bind, Butyrul CoEnzyme A <scene name='pdbligand=BCO:BUTYRYL+COENZYME+A'>(BCO)</scene> & 1,2-Ethanediol <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>(EDO)</scene>.

===Disease Relevance===

Since this enzyme is found in ''Homo sapiens'', or humans, and is a HAT Gcn5 bound to butyryl-CoA, the 5h86 enzyme has also been found to have a probable role in HIV-1 infections by being able to utilize the recruited viral protein "Tat" to regulate the activity that induces proviral gene chromatin remodeling <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity">PMID: 10607594</ref>. The Tat protein is responsible for the activation of the elongation stage of transcription by recruiting certain transcription factor complexes, like pTEFb, and coactivators like p300/CBP <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity"/>. Due to this correlation, the Tat protein may also have capabilities to promote HAT activity of the p300 coactivator which binds to the HIV-1 promoter region <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity"/>. Another similarity is that the HATs acetylate <scene name='80/806432/Lysine_residues/1'>lysine residues</scene> (Lys50 and Lys51), just like the 5h86 acetyltransferase <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity"/>. Due to this overall correlation, it is inferred that this 5h86 Gcn5 bound to butyryl-CoA may have a biochemical role in HIV-1 infections.

</StructureSection>

Sandbox Reserved 1546: Difference between revisions

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 20: / Line 20: @@
 == Structural Highlights & Disease ==
 <StructureSection load='1stp' size='340' side='right' caption='5H86 Structure' scene='80/806432/5h86_molecule/2'>
+===Structure===
 The enzyme, 5h86, can be expressed in ''E. coli'' and is found in humans <ref name = "Structural basis for acyl-group discrimination by human Gcn5L2"/>. The characteristics of this 5h86 Human Gcn5 enzyme bound to butyrl-coA can be summarized in the table below. Clicking on each green linked element will convey important structural information of the enzyme.
@@ Line 41: / Line 41: @@
 <tr id='activity'><td class="sblockLbl"><b>Type of Enzymatic Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histone_acetyltransferase Histone acetyltransferase]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://www.rcsb.org/pdb/explore.do?structureId=5h86 RCSB]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://www.rcsb.org/pdb/explore.do?structureId=5h86 RCSB 5h86 PDB], [http://proteopedia.org/wiki/index.php/5h86#cite_note-0 5h86 Proteopedia Page]</span></td></tr>
 </table></center>
@@ Line 47: / Line 47: @@
 <p>Under a "backbone" representation, the <scene name='80/806432/Unique_protein_chain/1'>one unique protein chain</scene> of the 5h86 enzyme can be visualized. The structure of this molecule is also composed of <scene name='80/806432/Secondary_structures/1'>alpha helices (magenta) and beta strands (gold)</scene>.</p>
-<p>The 168 <scene name='80/806432/Aa_residues/1'>amino acid residues</scene> can be visualized by the gold circles of the structure.</p>
+<p>The <scene name='80/806432/Aa_residues/3'>168 amino acid residues</scene> can be visualized by the green ball and stick structures of the molecule.</p>
 <p> There are <scene name='80/806432/Ligands/1'>two ligand binding sites</scene>, which can be represented in green and the protein structure is represented by a khaki color. The active site of Gcn5 contains two binding grooves where acetyl-CoA and peptide bind, Butyrul CoEnzyme A <scene name='pdbligand=BCO:BUTYRYL+COENZYME+A'>(BCO)</scene> & 1,2-Ethanediol <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>(EDO)</scene>.</p>
+===Disease Relevance===
+Since this enzyme is found in ''Homo sapiens'', or humans, and is a HAT Gcn5 bound to butyryl-CoA, the 5h86 enzyme has also been found to have a probable role in HIV-1 infections by being able to utilize the recruited viral protein "Tat" to regulate the activity that induces proviral gene chromatin remodeling <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity">PMID: 10607594</ref>. The Tat protein is responsible for the activation of the elongation stage of transcription by recruiting certain transcription factor complexes, like pTEFb, and coactivators like p300/CBP <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity"/>. Due to this correlation, the Tat protein may also have capabilities to promote HAT activity of the p300 coactivator which binds to the HIV-1 promoter region <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity"/>. Another similarity is that the HATs acetylate <scene name='80/806432/Lysine_residues/1'>lysine residues</scene> (Lys50 and Lys51), just like the 5h86 acetyltransferase <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity"/>. Due to this overall correlation, it is inferred that this 5h86 Gcn5 bound to butyryl-CoA may have a biochemical role in HIV-1 infections.
 </StructureSection>