Sandbox Reserved 1546: Difference between revisions

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== Structural Highlights & Disease ==

===Structure===

The enzyme, 5h86, can be expressed in ''E. coli'' and is found in humans <ref name = "Structural basis for acyl-group discrimination by human Gcn5L2"/>. The characteristics of this 5h86 Human Gcn5 enzyme bound to butyrl-coA can be summarized in the table below. Clicking on each green linked element will convey important structural information of the enzyme.

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<tr id='activity'><td class="sblockLbl">Type of Enzymatic Activity:</td><td class="sblockDat">[http://en.wikipedia.org/wiki/Histone_acetyltransferase Histone acetyltransferase]</td></tr>

<tr id='resources'><td class="sblockLbl">Resources:</td><td class="sblockDat">[http://www.rcsb.org/pdb/explore.do?structureId=5h86 RCSB 5h86 PDB][http://proteopedia.org/wiki/index.php/5h86#cite_note-0 5h86 Proteopedia Page]</td></tr>

<tr id='resources'><td class="sblockLbl">Resources:</td><td class="sblockDat">[http://www.rcsb.org/pdb/explore.do?structureId=5h86 RCSB 5h86 PDB], [http://proteopedia.org/wiki/index.php/5h86#cite_note-0 5h86 Proteopedia Page]</td></tr>

</table></center>

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===Disease Relevance===

Since this enzyme is found in ''Homo sapiens'', or humans, and is a Gcn5 bound to butyryl-CoA, the 5h86 enzyme has also been found to have a role in HIV-1 infections by being able to utilize the recruited viral protein "Tat" to regulate the activity that induces proviral gene chromatin remodeling <ref name = "~~Glucocorticoid~~-~~stimulated preadipocyte differentiation~~ is ~~mediated through acetylation of C/EBPβ by GCN5~~">~~doi~~: 10.~~1073~~/~~pnas.0607378104~~</~~ref~~><ref name = "~~The Double~~-~~Histone-Acetyltransferase Complex ATAC Is Essential~~ for ~~Mammalian Development~~">~~doi: 10~~.~~1128~~/~~MCB.01599-08~~</ref>.

Since this enzyme is found in ''Homo sapiens'', or humans, and is a HAT Gcn5 bound to butyryl-CoA, the 5h86 enzyme has also been found to have a probable role in HIV-1 infections by being able to utilize the recruited viral protein "Tat" to regulate the activity that induces proviral gene chromatin remodeling <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity">PMID: 10607594</ref>. The Tat protein is responsible for the activation of the elongation stage of transcription by recruiting certain transcription factor complexes, like pTEFb, and coactivators like p300/CBP <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity"/>. Due to this correlation, the Tat protein may also have capabilities to promote HAT activity of the p300 coactivator which binds to the HIV-1 promoter region <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity"/>. Another similarity is that the HATs acetylate <scene name='80/806432/Lysine_residues/1'>lysine residues</scene> (Lys50 and Lys51), just like the 5h86 acetyltransferase <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity"/>. Due to this overall correlation, it is inferred that this 5h86 Gcn5 bound to butyryl-CoA may have a biochemical role in HIV-1 infections.

</StructureSection>

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 == Structural Highlights & Disease ==
 <StructureSection load='1stp' size='340' side='right' caption='5H86 Structure' scene='80/806432/5h86_molecule/2'>
 ===Structure===
 The enzyme, 5h86, can be expressed in ''E. coli'' and is found in humans <ref name = "Structural basis for acyl-group discrimination by human Gcn5L2"/>. The characteristics of this 5h86 Human Gcn5 enzyme bound to butyrl-coA can be summarized in the table below. Clicking on each green linked element will convey important structural information of the enzyme.
@@ Line 42: / Line 41: @@
 <tr id='activity'><td class="sblockLbl"><b>Type of Enzymatic Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histone_acetyltransferase Histone acetyltransferase]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://www.rcsb.org/pdb/explore.do?structureId=5h86 RCSB 5h86 PDB][http://proteopedia.org/wiki/index.php/5h86#cite_note-0 5h86 Proteopedia Page]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://www.rcsb.org/pdb/explore.do?structureId=5h86 RCSB 5h86 PDB], [http://proteopedia.org/wiki/index.php/5h86#cite_note-0 5h86 Proteopedia Page]</span></td></tr>
 </table></center>
@@ Line 53: / Line 52: @@
 ===Disease Relevance===
-Since this enzyme is found in ''Homo sapiens'', or humans, and is a Gcn5 bound to butyryl-CoA, the 5h86 enzyme has also been found to have a role in HIV-1 infections by being able to utilize the recruited viral protein "Tat" to regulate the activity that induces proviral gene chromatin remodeling <ref name = "Glucocorticoid-stimulated preadipocyte differentiation is mediated through acetylation of C/EBPβ by GCN5">doi: 10.1073/pnas.0607378104</ref><ref name = "The Double-Histone-Acetyltransferase Complex ATAC Is Essential for Mammalian Development">doi: 10.1128/MCB.01599-08</ref>.
+Since this enzyme is found in ''Homo sapiens'', or humans, and is a HAT Gcn5 bound to butyryl-CoA, the 5h86 enzyme has also been found to have a probable role in HIV-1 infections by being able to utilize the recruited viral protein "Tat" to regulate the activity that induces proviral gene chromatin remodeling <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity">PMID: 10607594</ref>. The Tat protein is responsible for the activation of the elongation stage of transcription by recruiting certain transcription factor complexes, like pTEFb, and coactivators like p300/CBP <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity"/>. Due to this correlation, the Tat protein may also have capabilities to promote HAT activity of the p300 coactivator which binds to the HIV-1 promoter region <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity"/>. Another similarity is that the HATs acetylate <scene name='80/806432/Lysine_residues/1'>lysine residues</scene> (Lys50 and Lys51), just like the 5h86 acetyltransferase <ref name = "Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity"/>. Due to this overall correlation, it is inferred that this 5h86 Gcn5 bound to butyryl-CoA may have a biochemical role in HIV-1 infections.
 </StructureSection>