6or2: Difference between revisions

Latest revision as of 14:52, 13 March 2024

MmpL3 is a lipid transporter that binds trehalose monomycolate and phosphatidylethanolamine

Structural highlights

6or2 is a 1 chain structure with sequence from Mycolicibacterium smegmatis MC2 155. This structure supersedes the now removed PDB entry 6n3t. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.59Å
Ligands:	L9Q, LMT
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MMPL3_MYCS2 Transports trehalose monomycolate (TMM) to the cell wall (PubMed:31239378, PubMed:22520756, PubMed:28698380). Flips TMM across the inner membrane. Membrane potential is not required for this function (PubMed:28698380). Transports probably phosphatidylethanolamine (PE) as well. Binds specifically both TMM and PE, but not trehalose dimycolate (TDM). Binds also diacylglycerol (DAG) and other phospholipids, including phosphatidylglycerol (PG), phosphatidylinositol (PI), and cardiolipin (CDL) (PubMed:31113875). Contributes to membrane potential, cell wall composition, antibiotic susceptibility and fitness (PubMed:28703701).^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Varela C, Rittmann D, Singh A, Krumbach K, Bhatt K, Eggeling L, Besra GS, Bhatt A. MmpL genes are associated with mycolic acid metabolism in mycobacteria and corynebacteria. Chem Biol. 2012 Apr 20;19(4):498-506. doi: 10.1016/j.chembiol.2012.03.006. PMID:22520756 doi:https://dx.doi.org/10.1016/j.chembiol.2012.03.006
↑ Xu Z, Meshcheryakov VA, Poce G, Chng SS. MmpL3 is the flippase for mycolic acids in mycobacteria. Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):7993-7998. doi:, 10.1073/pnas.1700062114. Epub 2017 Jul 11. PMID:28698380 doi:https://dx.doi.org/10.1073/pnas.1700062114
↑ McNeil MB, Dennison D, Parish T. Mutations in MmpL3 alter membrane potential, hydrophobicity and antibiotic susceptibility in Mycobacterium smegmatis. Microbiology (Reading). 2017 Jul;163(7):1065-1070. doi: 10.1099/mic.0.000498., Epub 2017 Jul 21. PMID:28703701 doi:https://dx.doi.org/10.1099/mic.0.000498
↑ Su CC, Klenotic PA, Bolla JR, Purdy GE, Robinson CV, Yu EW. MmpL3 is a lipid transporter that binds trehalose monomycolate and phosphatidylethanolamine. Proc Natl Acad Sci U S A. 2019 May 21. pii: 1901346116. doi:, 10.1073/pnas.1901346116. PMID:31113875 doi:https://dx.doi.org/10.1073/pnas.1901346116
↑ Fay A, Czudnochowski N, Rock JM, Johnson JR, Krogan NJ, Rosenberg O, Glickman MS. Two Accessory Proteins Govern MmpL3 Mycolic Acid Transport in Mycobacteria. mBio. 2019 Jun 25;10(3). pii: mBio.00850-19. doi: 10.1128/mBio.00850-19. PMID:31239378 doi:https://dx.doi.org/10.1128/mBio.00850-19

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Varela C, Rittmann D, Singh A, Krumbach K, Bhatt K, Eggeling L, Besra GS, Bhatt A. MmpL genes are associated with mycolic acid metabolism in mycobacteria and corynebacteria. Chem Biol. 2012 Apr 20;19(4):498-506. doi: 10.1016/j.chembiol.2012.03.006. PMID:22520756 doi:https://dx.doi.org/10.1016/j.chembiol.2012.03.006

[2] Xu Z, Meshcheryakov VA, Poce G, Chng SS. MmpL3 is the flippase for mycolic acids in mycobacteria. Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):7993-7998. doi:, 10.1073/pnas.1700062114. Epub 2017 Jul 11. PMID:28698380 doi:https://dx.doi.org/10.1073/pnas.1700062114

[3] McNeil MB, Dennison D, Parish T. Mutations in MmpL3 alter membrane potential, hydrophobicity and antibiotic susceptibility in Mycobacterium smegmatis. Microbiology (Reading). 2017 Jul;163(7):1065-1070. doi: 10.1099/mic.0.000498., Epub 2017 Jul 21. PMID:28703701 doi:https://dx.doi.org/10.1099/mic.0.000498

[4] Su CC, Klenotic PA, Bolla JR, Purdy GE, Robinson CV, Yu EW. MmpL3 is a lipid transporter that binds trehalose monomycolate and phosphatidylethanolamine. Proc Natl Acad Sci U S A. 2019 May 21. pii: 1901346116. doi:, 10.1073/pnas.1901346116. PMID:31113875 doi:https://dx.doi.org/10.1073/pnas.1901346116

[5] Fay A, Czudnochowski N, Rock JM, Johnson JR, Krogan NJ, Rosenberg O, Glickman MS. Two Accessory Proteins Govern MmpL3 Mycolic Acid Transport in Mycobacteria. mBio. 2019 Jun 25;10(3). pii: mBio.00850-19. doi: 10.1128/mBio.00850-19. PMID:31239378 doi:https://dx.doi.org/10.1128/mBio.00850-19

[1]

[2]

[3]

[4]

[5]

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6or2 is ON HOLD
+==MmpL3 is a lipid transporter that binds trehalose monomycolate and phosphatidylethanolamine==
+<StructureSection load='6or2' size='340' side='right'caption='[[6or2]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
-Authors: Su, C.-C.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6or2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6n3t 6n3t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OR2 FirstGlance]. <br>
-Description: Crystal Structure of a membrane protein
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=L9Q:(1S)-2-{[(S)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(OCTADECANOYLOXY)METHYL]ETHYL+(9Z)-OCTADEC-9-ENOATE'>L9Q</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene></td></tr>
-[[Category: Su, C.-C]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6or2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6or2 OCA], [https://pdbe.org/6or2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6or2 RCSB], [https://www.ebi.ac.uk/pdbsum/6or2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6or2 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MMPL3_MYCS2 MMPL3_MYCS2] Transports trehalose monomycolate (TMM) to the cell wall (PubMed:31239378, PubMed:22520756, PubMed:28698380). Flips TMM across the inner membrane. Membrane potential is not required for this function (PubMed:28698380). Transports probably phosphatidylethanolamine (PE) as well. Binds specifically both TMM and PE, but not trehalose dimycolate (TDM). Binds also diacylglycerol (DAG) and other phospholipids, including phosphatidylglycerol (PG), phosphatidylinositol (PI), and cardiolipin (CDL) (PubMed:31113875). Contributes to membrane potential, cell wall composition, antibiotic susceptibility and fitness (PubMed:28703701).<ref>PMID:22520756</ref> <ref>PMID:28698380</ref> <ref>PMID:28703701</ref> <ref>PMID:31113875</ref> <ref>PMID:31239378</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Mycolicibacterium smegmatis MC2 155]]
+[[Category: Su C-C]]

6or2: Difference between revisions

Latest revision as of 14:52, 13 March 2024

MmpL3 is a lipid transporter that binds trehalose monomycolate and phosphatidylethanolamine

Structural highlights

Function

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools