4urp

The Crystal structure of Nitroreductase from Saccharomyces cerevisiae

spinqualitylabelsall models 4urp, resolution 2.99Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4urp is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.991Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function FRM2_YEAST Involved in negative regulation of fatty acid metabolism. Publication Abstract from PubMed Nitroreductases are flavoenzymes that catalyze nitrocompounds and are widely utilized in industrial applications due to their detoxification potential and activation of bio-medicinal pro-drugs. Type I nitroreductases are classified into subgroups depending on the use of NADPH or NADH as the electron donor. Here, we report the crystal structure of the fungal nitroreductase Frm2 from Saccharomyces cerevisiae, one of the uncharacterized subgroups of proteins, to reveal its minimal architecture previously observed in bacterial nitroreductases such as CinD and YdjA. The structure lacks protruding helical motifs that form part of the cofactor and substrate binding site, resulting in an open and wide active site geometry. Arg82 is uniquely conserved in proximity to the substrate binding site in Frm2 homologues and plays a crucial role in the activity of the active site. Frm2 primarily utilizes NADH to reduce 4-NQO. Because missing helical elements are involved in the direct binding to the NAD(P)H in group A or group B in Type I family, Frm2 and its homologues may represent a distinctive subgroup with an altered binding mode for the reducing compound. This result provides a structural basis for the rational design of novel pro-drugs with the ability to reduce nitrogen-containing hazardous molecules. This article is protected by copyright. All rights reserved. Crystal structure of the fungal nitroreductase Frm2 from Saccharomyces cerevisiae.,Song HN, Jeong DG, Bang SY, Paek SH, Park BC, Park SG, Woo EJ Protein Sci. 2015 Apr 11. doi: 10.1002/pro.2686. PMID:25864423 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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