6jxd: Difference between revisions

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'''Unreleased structure'''


The entry 6jxd is ON HOLD  until Paper Publication
==Human nucleosome core particle with cohesive end DNA termini==
<StructureSection load='6jxd' size='340' side='right'caption='[[6jxd]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6jxd]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JXD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JXD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jxd OCA], [https://pdbe.org/6jxd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jxd RCSB], [https://www.ebi.ac.uk/pdbsum/6jxd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jxd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/H31_HUMAN H31_HUMAN]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The poly(ADP-ribose) polymerase, PARP1, plays a key role in maintaining genomic integrity by detecting DNA damage and mediating repair. gammaH2A.X is the primary histone marker for DNA double-strand breaks and PARP1 localizes to H2A.X-enriched chromatin damage sites, but the basis for this association is not clear. We characterize the kinetics of PARP1 binding to a variety of nucleosomes harbouring DNA double-strand breaks, which reveal that PARP1 associates faster with (gamma)H2A.X- versus H2A-nucleosomes, resulting in a higher affinity for the former, which is maximal for gammaH2A.X-nucleosome that is also the activator eliciting the greatest poly-ADP-ribosylation catalytic efficiency. The enhanced activities with gammaH2A.X-nucleosome coincide with increased accessibility of the DNA termini resulting from the H2A.X-Ser139 phosphorylation. Indeed, H2A- and (gamma)H2A.X-nucleosomes have distinct stability characteristics, which are rationalized by mutational analysis and (gamma)H2A.X-nucleosome core crystal structures. This suggests that the gammaH2A.X epigenetic marker directly facilitates DNA repair by stabilizing PARP1 association and promoting catalysis.


Authors: DeFalco, L., Davey, C.A.
PARP1 exhibits enhanced association and catalytic efficiency with gammaH2A.X-nucleosome.,Sharma D, De Falco L, Padavattan S, Rao C, Geifman-Shochat S, Liu CF, Davey CA Nat Commun. 2019 Dec 17;10(1):5751. doi: 10.1038/s41467-019-13641-0. PMID:31848352<ref>PMID:31848352</ref>


Description: Human nucleosome core particle with cohesive end DNA termini
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Davey, C.A]]
<div class="pdbe-citations 6jxd" style="background-color:#fffaf0;"></div>
[[Category: Defalco, L]]
 
==See Also==
*[[Histone 3D structures|Histone 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Davey CA]]
[[Category: DeFalco L]]
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