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{{STRUCTURE_2zci|  PDB=2zci  |  SCENE=  }}
'''Structure of a GTP-dependent bacterial PEP-carboxykinase from Corynebacterium glutamicum'''


==Structure of a GTP-dependent bacterial PEP-carboxykinase from Corynebacterium glutamicum==
<StructureSection load='2zci' size='340' side='right'caption='[[2zci]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2zci]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZCI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zci OCA], [https://pdbe.org/2zci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zci RCSB], [https://www.ebi.ac.uk/pdbsum/2zci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zci ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PCKG_CORGL PCKG_CORGL] Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.<ref>PMID:11565516</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zc/2zci_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zci ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
GTP-dependent phosphoenolpyruvate carboxykinase (PCK) is the key enzyme that controls the blood glucose level during fasting in higher animals. Here we report the first substrate-free structure of a GTP-dependent phosphoenolpyruvate (PEP) carboxykinase from a bacterium, Corynebacterium glutamicum (CgPCK). The protein crystallizes in space group P2(1) with four molecules per asymmetric unit. The 2.3A resolution structure was solved by molecular replacement using the human cytosolic PCK (hcPCK) structure (PDB ID: 1KHF) as the starting model. The four molecules in the asymmetric unit pack as two dimers, and is an artifact of crystal packing. However, the P-loop and the guanine binding loop of the substrate-free CgPCK structure have different conformations from the other published GTP-specific PCK structures, which all have bound substrates and/or metal ions. It appears that a change in the P-loop and guanine binding loop conformation is necessary for substrate binding in GTP-specific PCKs, as opposed to overall domain movement in ATP-specific PCKs.


==Overview==
Structure of a GTP-dependent bacterial PEP-carboxykinase from Corynebacterium glutamicum.,Aich S, Prasad L, Delbaere LT Int J Biochem Cell Biol. 2008;40(8):1597-603. Epub 2007 Dec 23. PMID:18234538<ref>PMID:18234538</ref>
GTP-dependent phosphoenolpyruvate carboxykinase (PCK) is the key enzyme that controls the blood glucose level during fasting in higher animals. Here we report the first substrate-free structure of a GTP-dependent phosphoenolpyruvate (PEP) carboxykinase from a bacterium, Corynebacterium glutamicum (CgPCK). The protein crystallizes in space group P2(1) with four molecules per asymmetric unit. The 2.3A resolution structure was solved by molecular replacement using the human cytosolic PCK (hcPCK) structure (PDB ID: 1KHF) as the starting model. The four molecules in the asymmetric unit pack as two dimers, and is an artifact of crystal packing. However, the P-loop and the guanine binding loop of the substrate-free CgPCK structure have different conformations from the other published GTP-specific PCK structures, which all have bound substrates and/or metal ions. It appears that a change in the P-loop and guanine binding loop conformation is necessary for substrate binding in GTP-specific PCKs, as opposed to overall domain movement in ATP-specific PCKs.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2ZCI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCI OCA].
</div>
<div class="pdbe-citations 2zci" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structure of a GTP-dependent Bacterial PEP-carboxykinase from Corynebacterium glutamicum., Aich S, Prasad L, Delbaere LT, Int J Biochem Cell Biol. 2007 Dec 23;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18234538 18234538]
*[[Phosphoenolpyruvate carboxykinase 3D structures|Phosphoenolpyruvate carboxykinase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Corynebacterium glutamicum]]
[[Category: Corynebacterium glutamicum]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Aich, S.]]
[[Category: Aich S]]
[[Category: Delbaere, L T.J.]]
[[Category: Delbaere LTJ]]
[[Category: Prasad, L.]]
[[Category: Prasad L]]
[[Category: Carboxykinase]]
[[Category: Gtp-dependent]]
[[Category: Lyase]]
[[Category: Signaling protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 16 23:11:08 2008''

Latest revision as of 12:00, 30 August 2023

Structure of a GTP-dependent bacterial PEP-carboxykinase from Corynebacterium glutamicum

2zci, resolution 2.30Å

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