6e1j: Difference between revisions

Latest revision as of 06:17, 11 October 2023

Crystal Structure of Methylthioalkylmalate Synthase (BjuMAM1.1) from Brassica juncea

Structural highlights

6e1j is a 2 chain structure with sequence from Brassica juncea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.096Å
Ligands:	COA, KMT, MN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C5J4P1_BRAJU

Publication Abstract from PubMed

Methylthioalkylmalate synthase catalyzes the committed step in the side-chain elongation of methionine-derived aliphatic glucosinolates and likely evolved from the isopropylmalate synthases of leucine biosynthesis. The globally cultivated Brassica species possess diverse aliphatic glucosinolates important for plant defense and animal nutrition; however, the molecular basis for the evolution of methylthioalkylmalate synthase and its generation of natural product diversity in Brassica is poorly understood. Here we show that Brassica genomes encode multiple methylthioalkylmalate synthase that have differences in expression profiles and 2-oxo substrate preference that account for diversity of aliphatic glucosinolates across Brassica accessions. The 2.1 A resolution x-ray crystal structure of B. juncea methylthioalkylmalate synthase identifies key active site residues responsible for controlling specificity for different 2-oxo substrates and the determinants of side-chain length in aliphatic glucosinolates. Overall, these results provide the evolutionary and biochemical foundation for diversification of glucosinolates profiles across globally-cultivated Brassica species, which could be used with ongoing breeding strategies towards manipulation of beneficial glucosinolates compounds for animal health and plant protection.

Molecular Basis of the Evolution of Methylthioalkylmalate Synthase and Diversity of Methionine-Derived Glucosinolates.,Kumar R, Lee SG, Augustine R, Reichelt M, Vassao DG, Palavalli MH, Allen A, Gershenzon J, Jez JM, Bisht NC Plant Cell. 2019 Apr 25. pii: tpc.19.00046. doi: 10.1105/tpc.19.00046. PMID:31023839^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kumar R, Lee SG, Augustine R, Reichelt M, Vassao DG, Palavalli MH, Allen A, Gershenzon J, Jez JM, Bisht NC. Molecular Basis of the Evolution of Methylthioalkylmalate Synthase and Diversity of Methionine-Derived Glucosinolates. Plant Cell. 2019 Apr 25. pii: tpc.19.00046. doi: 10.1105/tpc.19.00046. PMID:31023839 doi:https://dx.doi.org/10.1105/tpc.19.00046

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OCA

[1] Kumar R, Lee SG, Augustine R, Reichelt M, Vassao DG, Palavalli MH, Allen A, Gershenzon J, Jez JM, Bisht NC. Molecular Basis of the Evolution of Methylthioalkylmalate Synthase and Diversity of Methionine-Derived Glucosinolates. Plant Cell. 2019 Apr 25. pii: tpc.19.00046. doi: 10.1105/tpc.19.00046. PMID:31023839 doi:https://dx.doi.org/10.1105/tpc.19.00046

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='6e1j' size='340' side='right'caption='[[6e1j]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6e1j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Braju Braju]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E1J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E1J FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6e1j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Brassica_juncea Brassica juncea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E1J FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=KMT:4-(METHYLSULFANYL)-2-OXOBUTANOIC+ACID'>KMT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.096&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gsl-elong-A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3707 BRAJU])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=KMT:4-(METHYLSULFANYL)-2-OXOBUTANOIC+ACID'>KMT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e1j OCA], [http://pdbe.org/6e1j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e1j RCSB], [http://www.ebi.ac.uk/pdbsum/6e1j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e1j ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e1j OCA], [https://pdbe.org/6e1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e1j RCSB], [https://www.ebi.ac.uk/pdbsum/6e1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e1j ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/C5J4P1_BRAJU C5J4P1_BRAJU]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 19: @@
 </div>
 <div class="pdbe-citations 6e1j" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[2-isopropylmalate synthase 3D structures|2-isopropylmalate synthase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Braju]]
+[[Category: Brassica juncea]]
 [[Category: Large Structures]]
-[[Category: Jez, J M]]
+[[Category: Jez JM]]
-[[Category: Lee, S G]]
+[[Category: Lee SG]]
-[[Category: Enzyme]]
-[[Category: Methylthioalkylmalate synthase]]
-[[Category: Plant protein]]
-[[Category: X-ray crystal structure]]

6e1j: Difference between revisions

Latest revision as of 06:17, 11 October 2023

Crystal Structure of Methylthioalkylmalate Synthase (BjuMAM1.1) from Brassica juncea

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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