6rm3

Evolutionary compaction and adaptation visualized by the structure of the dormant microsporidian ribosome

Loading... Status: waiting for Mol* initialization Structural highlights 6rm3 is a 10 chain structure with sequence from Vairimorpha necatrix. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.4Å Ligands: MG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Microsporidia are eukaryotic parasites that infect essentially all animal species, including many of agricultural importance(1-3), and are significant opportunistic parasites of humans(4). They are characterized by having a specialized infection apparatus, an obligate intracellular lifestyle(5), rudimentary mitochondria and the smallest known eukaryotic genomes(5-7). Extreme genome compaction led to minimal gene sizes affecting even conserved ancient complexes such as the ribosome(8-10). In the present study, the cryo-electron microscopy structure of the ribosome from the microsporidium Vairimorpha necatrix is presented, which illustrates how genome compaction has resulted in the smallest known eukaryotic cytoplasmic ribosome. Selection pressure led to the loss of two ribosomal proteins and removal of essentially all eukaryote-specific ribosomal RNA (rRNA) expansion segments, reducing the rRNA to a functionally conserved core. The structure highlights how one microsporidia-specific and several repurposed existing ribosomal proteins compensate for the extensive rRNA reduction. The microsporidian ribosome is kept in an inactive state by two previously uncharacterized dormancy factors that specifically target the functionally important E-site, P-site and polypeptide exit tunnel. The present study illustrates the distinct effects of evolutionary pressure on RNA and protein-coding genes, provides a mechanism for ribosome inhibition and can serve as a structural basis for the development of inhibitors against microsporidian parasites. Evolutionary compaction and adaptation visualized by the structure of the dormant microsporidian ribosome.,Barandun J, Hunziker M, Vossbrinck CR, Klinge S Nat Microbiol. 2019 Jul 22. pii: 10.1038/s41564-019-0514-6. doi:, 10.1038/s41564-019-0514-6. PMID:31332387 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures

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