3c3w: Difference between revisions

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==Crystal Structure of the Mycobacterium tuberculosis Hypoxic Response Regulator DosR==
The line below this paragraph, containing "STRUCTURE_3c3w", creates the "Structure Box" on the page.
<StructureSection load='3c3w' size='340' side='right'caption='[[3c3w]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3c3w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C3W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C3W FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_3c3w| PDB=3c3w |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c3w OCA], [https://pdbe.org/3c3w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c3w RCSB], [https://www.ebi.ac.uk/pdbsum/3c3w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c3w ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DEVR_MYCTU DEVR_MYCTU] Member of the two-component regulatory system DevR/DevS (also called DosR/DosS) involved in onset of the dormancy response (PubMed:15033981). Regulates an approximately 48-member regulon (PubMed:12953092, PubMed:11416222, PubMed:15033981, PubMed:18400743). When phosphorylated binds and activates the promoter of DevR regulon genes in response to hypoxia (PubMed:18359816, PubMed:21764934, PubMed:28977726). The presence of target DNA increases stability of phospho-DevR in vitro (PubMed:28977726). Activates its own transcription under hypoxic but not aerobic conditions, probably binds as a dimer to tandem binding sites within the devR and hspX promoters (PubMed:18359816). Accepts a phosphate group from DevS (DosS) and from DosT (PubMed:15033981, PubMed:15073296, PubMed:21764934, PubMed:28977726). Does not regulate transcription of dosT (PubMed:19487478).<ref>PMID:11416222</ref> <ref>PMID:12953092</ref> <ref>PMID:15033981</ref> <ref>PMID:15073296</ref> <ref>PMID:18359816</ref> <ref>PMID:18400743</ref> <ref>PMID:19487478</ref> <ref>PMID:21764934</ref> <ref>PMID:28977726</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c3/3c3w_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3c3w ConSurf].
<div style="clear:both"></div>


'''Crystal Structure of the Mycobacterium tuberculosis Hypoxic Response Regulator DosR'''
==See Also==
 
*[[Response regulator 3D structure|Response regulator 3D structure]]
 
== References ==
==Overview==
<references/>
The response regulator DosR is essential for promoting long-term survival of Mycobacterium tuberculosis under low oxygen conditions in a dormant state and may be responsible for latent tuberculosis in one-third of the world's population. Here, we report crystal structures of full-length unphosphorylated DosR at 2.2 A resolution and its C-terminal DNA-binding domain at 1.7 A resolution. The full-length DosR structure reveals several features never seen before in other response regulators. The N-terminal domain of the full-length DosR structure has an unexpected (beta alpha)(4) topology instead of the canonical (beta alpha)(5) fold observed in other response regulators. The linker region adopts a unique conformation that contains two helices forming a four-helix bundle with two helices from another subunit, resulting in dimer formation. The C-terminal domain in the full-length DosR structure displays a novel location of helix alpha 10, which allows Gln199 to interact with the catalytic Asp54 residue of the N-terminal domain. In contrast, the structure of the DosR C-terminal domain alone displays a remarkable unstructured conformation for helix alpha 10 residues, different from the well-defined helical conformations in all other known structures, indicating considerable flexibility within the C-terminal domain. Our structures suggest a mode of DosR activation by phosphorylation via a helix rearrangement mechanism.
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
3C3W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C3W OCA].
 
==Reference==
Crystal structures of the response regulator DosR from Mycobacterium tuberculosis suggest a helix rearrangement mechanism for phosphorylation activation., Wisedchaisri G, Wu M, Sherman DR, Hol WG, J Mol Biol. 2008 Apr 18;378(1):227-42. Epub 2008 Feb 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18353359 18353359]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Single protein]]
[[Category: Hol WGJ]]
[[Category: Hol, W G.J.]]
[[Category: Sherman DR]]
[[Category: Sherman, D R.]]
[[Category: Wisedchaisri G]]
[[Category: Wisedchaisri, G.]]
[[Category: Wu M]]
[[Category: Wu, M.]]
[[Category: Dna-binding protein]]
[[Category: Response regulator]]
[[Category: Transcription]]
[[Category: Transcription regulation]]
[[Category: Tuberculosis]]
[[Category: Two-component regulatory system]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:51:47 2008''

Latest revision as of 09:32, 21 February 2024

Crystal Structure of the Mycobacterium tuberculosis Hypoxic Response Regulator DosR

3c3w, resolution 2.20Å

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