6kr1: Difference between revisions

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New page: '''Unreleased structure''' The entry 6kr1 is ON HOLD Authors: Ha, N.-C., Jeong, S. Description: ATP dependent protease HslV from Staphylococcus aureus [[Category: Unreleased Structures...
 
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'''Unreleased structure'''


The entry 6kr1 is ON HOLD
==ATP dependent protease HslV from Staphylococcus aureus==
<StructureSection load='6kr1' size='340' side='right'caption='[[6kr1]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6kr1]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KR1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KR1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kr1 OCA], [https://pdbe.org/6kr1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kr1 RCSB], [https://www.ebi.ac.uk/pdbsum/6kr1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kr1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HSLV_STAAM HSLV_STAAM] Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
HslUV is a bacterial heat shock protein complex consisting of the AAA+ ATPase component HslU and the protease component HslV. HslV is a threonine (Thr) protease employing the N-terminal Thr residue in the mature protein as the catalytic residue. To date, HslUV from Gram-negative bacteria has been extensively studied. However, the mechanisms of action and activation of HslUV from Gram-positive bacteria, which have an additional N-terminal sequence before the catalytic Thr residue, remain to be revealed. In this study, we determined the crystal structures of HslV from the Gram-positive bacterium Staphylococcus aureus with and without HslU in the crystallization conditions. The structural comparison suggested that a structural transition to the symmetric form of HslV was triggered by ATP-bound HslU. More importantly, the additional N-terminal sequence was cleaved in the presence of HslU and ATP, exposing the Thr9 residue at the N-terminus and activating the ATP-dependent protease activity. Further biochemical studies demonstrated that the exposed N-terminal Thr residue is critical for catalysis with binding to the symmetric HslU hexamer. Since eukaryotic proteasomes have a similar additional N-terminal sequence, our results will improve our understanding of the common molecular mechanisms for the activation of proteasomes.


Authors: Ha, N.-C., Jeong, S.
Cleavage-Dependent Activation of ATP-Dependent Protease HslUV from Staphylococcus aureus.,Jeong S, Ahn J, Kwon AR, Ha NC Mol Cells. 2020 Aug 31;43(8):694-704. doi: 10.14348/molcells.2020.0074. PMID:32694241<ref>PMID:32694241</ref>


Description: ATP dependent protease HslV from Staphylococcus aureus
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Ha, N.-C]]
<div class="pdbe-citations 6kr1" style="background-color:#fffaf0;"></div>
[[Category: Jeong, S]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus subsp. aureus Mu50]]
[[Category: Ha N-C]]
[[Category: Jeong S]]
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