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==Solution structure of stem-loop alpha of the hepatitis B virus post-transcriptional regulatory element==
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<StructureSection load='2jym' size='340' side='right'caption='[[2jym]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2jym]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JYM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JYM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jym OCA], [https://pdbe.org/2jym PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jym RCSB], [https://www.ebi.ac.uk/pdbsum/2jym PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jym ProSAT]</span></td></tr>
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<div style="background-color:#fffaf0;">
'''Solution structure of stem-loop alpha of the hepatitis B virus post-transcriptional regulatory element'''
== Publication Abstract from PubMed ==
 
 
==Overview==
Chronic hepatitis B virus (HBV) infections may lead to severe diseases like liver cirrhosis or hepatocellular carcinoma (HCC). The HBV post-transcriptional regulatory element (HPRE) facilitates the nuclear export of unspliced viral mRNAs, contains a splicing regulatory element and resides in the 3'-region of all viral transcripts. The HPRE consists of three sub-elements alpha (nucleotides 1151-1346), beta1 (nucleotides 1347-1457) and beta2 (nucleotides 1458-1582), which confer together full export competence. Here, we present the NMR solution structure (pdb 2JYM) of the stem-loop alpha (SLalpha, nucleotides 1292-1321) located in the sub-element alpha. The SLalpha contains a CAGGC pentaloop highly conserved in hepatoviruses, which essentially adopts a CUNG-like tetraloop conformation. Furthermore, the SLalpha harbours a single bulged G residue flanked by A-helical regions. The structure is highly suggestive of serving two functions in the context of export of unspliced viral RNA: binding sterile alpha motif (SAM-) domain containing proteins and/or preventing the utilization of a 3'-splice site contained within SLalpha.
Chronic hepatitis B virus (HBV) infections may lead to severe diseases like liver cirrhosis or hepatocellular carcinoma (HCC). The HBV post-transcriptional regulatory element (HPRE) facilitates the nuclear export of unspliced viral mRNAs, contains a splicing regulatory element and resides in the 3'-region of all viral transcripts. The HPRE consists of three sub-elements alpha (nucleotides 1151-1346), beta1 (nucleotides 1347-1457) and beta2 (nucleotides 1458-1582), which confer together full export competence. Here, we present the NMR solution structure (pdb 2JYM) of the stem-loop alpha (SLalpha, nucleotides 1292-1321) located in the sub-element alpha. The SLalpha contains a CAGGC pentaloop highly conserved in hepatoviruses, which essentially adopts a CUNG-like tetraloop conformation. Furthermore, the SLalpha harbours a single bulged G residue flanked by A-helical regions. The structure is highly suggestive of serving two functions in the context of export of unspliced viral RNA: binding sterile alpha motif (SAM-) domain containing proteins and/or preventing the utilization of a 3'-splice site contained within SLalpha.


==About this Structure==
Solution structure of stem-loop alpha of the hepatitis B virus post-transcriptional regulatory element.,Schwalbe M, Ohlenschlager O, Marchanka A, Ramachandran R, Hafner S, Heise T, Gorlach M Nucleic Acids Res. 2008 Mar;36(5):1681-9. Epub 2008 Feb 7. PMID:18263618<ref>PMID:18263618</ref>
2JYM is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JYM OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Solution structure of stem-loop alpha of the hepatitis B virus post-transcriptional regulatory element., Schwalbe M, Ohlenschlager O, Marchanka A, Ramachandran R, Hafner S, Heise T, Gorlach M, Nucleic Acids Res. 2008 Mar;36(5):1681-9. Epub 2008 Feb 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18263618 18263618]
</div>
[[Category: Single protein]]
<div class="pdbe-citations 2jym" style="background-color:#fffaf0;"></div>
[[Category: Gorlach, M.]]
== References ==
[[Category: Ohlenschlager, O.]]
<references/>
[[Category: Schwalbe, M.]]
__TOC__
[[Category: Hepatitis b virus]]
</StructureSection>
[[Category: Nmr solution structure]]
[[Category: Large Structures]]
[[Category: Post-transcriptional regulatory element]]
[[Category: Gorlach M]]
[[Category: Rna]]
[[Category: Ohlenschlager O]]
[[Category: Stem-loop alpha]]
[[Category: Schwalbe M]]
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